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Apc annexin 5

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APC-Annexin V is a fluorescent dye used in flow cytometry applications. It binds specifically to phosphatidylserine, which is externalized on the surface of apoptotic cells. This allows for the identification and quantification of apoptotic cells in a sample.

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Lab products found in correlation

Lsrfortessa flow cytometer, by BD (2 mentions) Fcs express 7 research edition, by De Novo Software (2 mentions) Annexin 5 fitc kit, by Miltenyi Biotec (2 mentions)

2 protocols using apc annexin 5

1

Comprehensive Cell Death Analysis by Flow Cytometry

Cited 1 time
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All flow cytometry was performed using LSRFortessa Flow Cytometer (BD Biosciences). GFP-linked shRNA competition assays were performed as described previously (75 (link)). Apoptosis was assayed using APC-Annexin V (Tonbo Biosciences) or Annexin V-FITC Kit (Miltenyi Biotec). All data were processed and analyzed with FCS Express 7 Research Edition (De Novo Software, Inc.; RRID:SCR_016431). All data were plotted with GraphPad (Prism, RRID:SCR_002798).
Patel A.J., Warda S., Maag J.L., Misra R., Miranda-Román M.A., Pachai M.R., Lee C.J., Li D., Wang N., Bayshtok G., Fishinevich E., Meng Y., Wong E.W., Yan J., Giff E., Pappalardi M.B., McCabe M.T., Fletcher J.A., Rudin C.M., Chandarlapaty S., Scandura J.M., Koche R.P., Glass J.L., Antonescu C.R., Zheng D., Chen Y, & Chi P. (2022). PRC2-Inactivating Mutations in Cancer Enhance Cytotoxic Response to DNMT1-Targeted Therapy via Enhanced Viral Mimicry. Cancer Discovery, 12(9), 2120-2139.
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2

Multiparametric Flow Cytometry Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All flow cytometry was performed using LSRFortessa Flow Cytometer (BD Biosciences). GFP linked shRNA competition assays were performed as described previously(80 (link)). Apoptosis was assayed using APC-Annexin V (Tonbo biosciences) or Annexin V-FITC Kit (Miltenyi Biotec). All data were processed and analyzed with FCS Express 7 Research Edition (De Novo Software, Inc; RRID:SCR_016431). All data plotted with GraphPad (Prism, RRID:SCR_002798).
Patel A.J., Warda S., Maag J.L., Misra R., Miranda-Román M.A., Pachai M.R., Lee C.J., Li D., Wang N., Bayshtok G., Fishinevich E., Meng Y., Wong E.W., Yan J., Giff E., Pappalardi M.B., McCabe M.T., Fletcher J.A., Rudin C.M., Chandarlapaty S., Scandura J.M., Koche R.P., Glass J.L., Antonescu C.R., Zheng D., Chen Y, & Chi P. (2022). PRC2-Inactivating Mutations in Cancer Enhance Cytotoxic Response to DNMT1-Targeted Therapy via Enhanced Viral Mimicry. Cancer Discovery, 12(9), 2120-2139.
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