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Mouse anti atg5 7c6

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The Mouse anti-Atg5 (7C6) is a monoclonal antibody that specifically binds to the Autophagy-related protein 5 (Atg5). Atg5 is a key component involved in the autophagy process, which is essential for the degradation and recycling of cellular components. This antibody can be used for the detection and analysis of Atg5 in various experimental applications.

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Lab products found in correlation

X ray film, by Kodak (2 mentions) Polyvinylidene fluoride (pvdf), by Merck Group (2 mentions) Lumiglo reagent, by Cell Signaling Technology (2 mentions) Rabbit anti β actin, by Cell Signaling Technology (2 mentions)

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Anti-ATG5 (2 citations)

2 protocols using mouse anti atg5 7c6

1

Immunoblot Analysis of Autophagy Proteins

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Whole cell lysates of indicated cell population were resolved on 4–15% SDS-PAGE and then transferred to a PVDF (polyvinylidene difluoride) membrane (Millipore). The membrane was blotted with rabbit anti-β-actin (13E5; Cell signaling), rabbit anti-Atg7 (catalog#A2856; Sigma-aldridge), mouse anti-Atg5 (7C6; Nanotools), rabbit anti-LC3 (catalog#L7543; Sigma-aldridge) or guinea pig anti-p62 (catalog#GP62-C; Progen Biotechnic and catalog#P0067; Sigma-aldridge); secondary HRP-linked antibodies were goat anti-rabbit (catalog#7074; Cell signaling), goat anti-mouse (catalog#1034–05; Southern Biotech) and goat anti-guinea pig (catalog#106–035–003; Jackson ImmunoResearch). The blots were developed in LumiGLO reagent (Cell signaling) prior to exposure to X-ray films (Kodak).
Xu X., Araki K., Li S., Han J.H., Ye L., Tan W.G., Konieczny B.T., Bruinsma M.W., Martinez J., Pearce E.L., Green D.R., Jones D.P., Virgin H.W, & Ahmed R. (2014). Autophagy is essential for effector CD8 T cell survival and memory formation. Nature immunology, 15(12), 1152-1161.
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2

Immunoblot Analysis of Autophagy Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell lysates of indicated cell population were resolved on 4–15% SDS-PAGE and then transferred to a PVDF (polyvinylidene difluoride) membrane (Millipore). The membrane was blotted with rabbit anti-β-actin (13E5; Cell signaling), rabbit anti-Atg7 (catalog#A2856; Sigma-aldridge), mouse anti-Atg5 (7C6; Nanotools), rabbit anti-LC3 (catalog#L7543; Sigma-aldridge) or guinea pig anti-p62 (catalog#GP62-C; Progen Biotechnic and catalog#P0067; Sigma-aldridge); secondary HRP-linked antibodies were goat anti-rabbit (catalog#7074; Cell signaling), goat anti-mouse (catalog#1034–05; Southern Biotech) and goat anti-guinea pig (catalog#106–035–003; Jackson ImmunoResearch). The blots were developed in LumiGLO reagent (Cell signaling) prior to exposure to X-ray films (Kodak).
Xu X., Araki K., Li S., Han J.H., Ye L., Tan W.G., Konieczny B.T., Bruinsma M.W., Martinez J., Pearce E.L., Green D.R., Jones D.P., Virgin H.W, & Ahmed R. (2014). Autophagy is essential for effector CD8 T cell survival and memory formation. Nature immunology, 15(12), 1152-1161.
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