For IHC analyses, paraffin-embedded samples from 113 serous-type ovarian cancer patients were immunostained with the anti-HSP70 antibody (1:500; CM407A; BioCare Medical). Then, horseradish peroxidase (HRP)-conjugated immunoglobulin G (IgG) antibody was added and incubated for 1 h, and the specimens were analyzed by using avidin and biotinylated enzyme complex (ABC) detection. The IHC images were observed under a ZEISS Axio Imager D2 microscope, and the nuclear HSP70 level was determined by using Nuance imaging software (version 3.0.2, PerkinElmer) based on three views of 200× field. This study was approved by the Human Experimental and Ethics Committee of NCKUH (IRB number A-ER-104-014) and Chi Mei Medical Center (IRB number 10807-005). Written informed consent for participation in the study was obtained from the participants.
Nuance imaging software
Manufactured by PerkinElmer
Sourced in United States
Nuance imaging software is a powerful tool for analyzing and visualizing microscopic images. It provides a range of image processing and analysis capabilities, including image segmentation, object detection, and quantification. The software can be used with a variety of imaging modalities, including fluorescence, brightfield, and phase contrast microscopy.
Automatically generated - may contain errors
2 protocols using nuance imaging software
1
Quantifying Nuclear HSP70 in Ovarian Cancer
2
Immunohistochemical analysis of trophoblast cells
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Formalin-fixed paraffin-embedded tissue samples were obtained and stained with anti-human CK7 antibody (1:500 dilution; ab183344; Abcam, Inc., Cambridge, UK) as the positive control for trophoblast cells. Anti-human SIK3 antibody (1:100 dilution; Dr. provided by Neng-Yao Shih), anti-human CD68 antibody (1:500 dilution; M0814; Agilent Technologies, Inc., Santa Clara, CA, USA), or anti-human CD204 antibody (1:200 dilution; MAB1710; Abnova Corporation) was used to stain the trophoblast cells and macrophages near the decidua. The sections were serially dewaxed, rehydrated, and heated with 10 mM sodium citrate (pH 6.0) for 20 min for antigen retrieval. After endogenous peroxidases were blocked with 3% hydrogen peroxide, the sections were incubated with the primary antibody overnight at 4 °C. Horseradish peroxidase (HRP)-conjugated immunoglobulin G (IgG) antibody was added and incubated for 1 h, and the specimens were analyzed through avidin and biotinylated enzyme complex (ABC) detection. Images were obtained under a ZEISS Axio Imager D2 microscope, and quantification (%Area) based on three 200× fields was performed using Nuance imaging software (version 3.0.2, PerkinElmer, Burlington, VT, USA).
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