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2 protocols using l cysteine and l glutamine 21013

1

Multiomics Analysis of Cell Lines

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All cell lines were purchased from ATCC including HeLa (ATCC CCL-2), Raw264.7 (ATCC TIB-71), MDA-MB-231 (ATCC HTB-26), MDA-MB-468(ATCC HTB-132), MCF7 (ATCC HTB-22), 3T3-L1 MBX (ATCC CRL-3242), U-87 MG (ATCC HTB-14). Azido-palmitic acid (1346) and L-Azidohomoalanine (1066) were purchased from Click chemistry tools. D-glucose (1,2,3,4,5,6,6-D7, 97–98%, DLM-2062), algal amino acid mixture (U-13C, 97–99%, CLM-1548), 2-deoxy-D-glucose (U-13C6, 99%, CLM-10466) were purchased from Cambridge isotope laboratories. Deuterium oxide (151882), Taxol (T7402) and Gemcitabine (G6423) were purchased from Sigma-Aldrich. DMEM medium (11965), FBS (10082), penicillin/streptomycin (1514), DMEM medium without L-methionine, L-cysteine and L-glutamine (21013), DMEM without glucose (11966) and proteinase K (EO0491) were purchased from ThermoFisher Scientific. CaF2 substrates (CAFP25–1, CAFP13–1 and CAFP-76–26-1U) were purchased from Crystran.
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2

Multiomics Analysis of Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
All cell lines were purchased from ATCC including HeLa (ATCC CCL-2), Raw264.7 (ATCC TIB-71), MDA-MB-231 (ATCC HTB-26), MDA-MB-468(ATCC HTB-132), MCF7 (ATCC HTB-22), 3T3-L1 MBX (ATCC CRL-3242), U-87 MG (ATCC HTB-14). Azido-palmitic acid (1346) and L-Azidohomoalanine (1066) were purchased from Click chemistry tools. D-glucose (1,2,3,4,5,6,6-D7, 97–98%, DLM-2062), algal amino acid mixture (U-13C, 97–99%, CLM-1548), 2-deoxy-D-glucose (U-13C6, 99%, CLM-10466) were purchased from Cambridge isotope laboratories. Deuterium oxide (151882), Taxol (T7402) and Gemcitabine (G6423) were purchased from Sigma-Aldrich. DMEM medium (11965), FBS (10082), penicillin/streptomycin (1514), DMEM medium without L-methionine, L-cysteine and L-glutamine (21013), DMEM without glucose (11966) and proteinase K (EO0491) were purchased from ThermoFisher Scientific. CaF2 substrates (CAFP25–1, CAFP13–1 and CAFP-76–26-1U) were purchased from Crystran.
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