The following antibodies were used: FLAG (Sigma, #F7425), KMT2D (Bethyl Laboratories, #A300-BL1185), UTX (Bethyl Laboratories, #A302–374A), ASH2L (Bethyl Laboratories, #A300–489A), WDR5 (Bethyl Laboratories, #A302–430A), DPY30 (Bethyl Laboratories, #A304–296A), NCOA6 (Bethyl Laboratories, #A300–411A), CUL1 (Invitrogen, #718700), FBXW7 (Bethyl Laboratories, #A301–720), C-MYC (Santa Crus, #sc-764), c-MYC-pT58 (Applied Biological Materials, #Y011034), HA (Biolegend, #901513), HA (Cell Signaling Technology, #3724), α-Tubulin (Santa Crus, #sc-8035), Skp1 (Santa Cruz, #sc-7163), CyclinE1 (Cell Signaling Technology, #4129), Cdk2 (Santa Cruz, #sc-6248), CyclinE (clone HE12), Vinculin (Santa Cruz, #sc-73614), ubiquitin K48 (EMD Millipore, 05–1307), Rabbit IgG-HRP (GE Healthcare, NA934V), mouse IgG-HRP (GE Healthcare, NA931V) and anti-FLAG M2 Affinity Gel (Sigma, #A2220).
Ncoa6
Manufactured by Fortis Life Sciences
NCOA6 is a laboratory equipment product that serves as a nuclear receptor coactivator. It functions to enhance the transcriptional activity of various nuclear receptors.
Automatically generated - may contain errors
Lab products found in correlation
Fbxw7, by Fortis Life Sciences (1 mentions)
Vinculin, by Santa Cruz Biotechnology (1 mentions)
K48 ubiquitin, by Merck Group (1 mentions)
Flag tag (flag), by Merck Group (1 mentions)
Anti flag m2 affinity gel, by Merck Group (1 mentions)
Dpy30, by Fortis Life Sciences (1 mentions)
Ash2l, by Fortis Life Sciences (1 mentions)
Cyclin e, by Santa Cruz Biotechnology (1 mentions)
Cyclin e1, by Cell Signaling Technology (1 mentions)
Cell lysis buffer, by Beyotime (1 mentions)
E cadherin, by Proteintech (1 mentions)
N cadherin, by Proteintech (1 mentions)
Chemidoc xps system, by Bio-Rad (1 mentions)
Bca protein assay kit, by Abcam (1 mentions)
β actin, by Proteintech (1 mentions)
Trans blot turbo system, by Bio-Rad (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
2 protocols using ncoa6
1
Immunoblotting and Immunoprecipitation Protocol
2
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were collected, washed once with ice‐cold PBS, and lysed on ice for 30 min using a cell lysis buffer (p0013; Beyotime) according to the manufacturer’s protocol. Protein quantification was undertaken using a BCA Protein Assay Kit (ab102536; Abcam). A total of 30 μg protein was denatured in loading buffer at 95°C for 5 min, and western blotting was carried out using a Bio‐Rad system (TGX 10%–15% gels). Transfer onto PVDF membranes was undertaken using a Trans‐Blot turbo system (Bio‐Rad). Images were acquired using the Bio‐Rad Imaging ChemiDoc XPS+ system. Secondary anti‐rabbit and anti‐mouse HRP‐conjugated Abs were purchased from Beyotime (A0208 and A0216, respectively). Proteins were detected using the following Abs: NCOA6 (A300‐410A; Bethyl), E‐cadherin (20874‐1‐AP; Proteintech), N‐cadherin (22018‐1‐AP; Proteintech), vimentin (5741; Cell Signaling Technology), and β‐actin (20536‐1‐AP; Proteintech).
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