MG132-treated transfected cells were collected and treated overnight with Protein-A/G. MagBeads (Yeasen) and anti-ELAVL1 (Cell Signaling Technology) or anti-β-TrCP (Cell Signaling Technology) antibodies at 4 °C. The immune complexes were centrifuged and washed, and the proteins were assessed by western blotting with anti-ELAVL1 or anti-βTrCP antibodies [24 (link)].
Anti elavl1
Manufactured by Cell Signaling Technology
Anti-ELAVL1 is a laboratory reagent that can be used to detect the ELAVL1 protein. ELAVL1 is an RNA-binding protein involved in the regulation of gene expression. Anti-ELAVL1 can be used in various experimental techniques, such as Western blotting and immunoprecipitation, to study the expression and function of ELAVL1 in biological systems.
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2 protocols using anti elavl1
1
Immunoprecipitation and Western Blot Analysis
2
RIP Assay for ELAVL1 mRNA Targets
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RIP assays were performed by using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer’s instructions. Briefly, cells were lysed in lysis buffer and the cleared lysates were immunoprecipitated with the indicated anti-ELAVL1 and anti-IgG antibodies (Cell Signaling Technology). Immunoprecipitated and input RNA was isolated and reverse transcribed for qRT-PCR amplifications with PTEN 3′-UTR-specific primers. mRNA relative expression level was normalized to input mRNA expression. The primers used for amplification are listed in Supplementary Table 1 .
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