Db wax usn 125 7031 column

Chromatographic analysis was carried out in a 6890N Network GC system (Agilent, Palo Alto, CA, USA) coupled to an MS Agilent technologies 5973 Network selective detector (Thermo Fischer Scientific, Waltham, MA, USA). Helium was used as a carrier gas. Separations were accomplished in a DB Wax USN 125-7031 column (30 m × 0.25 mm × 0.25 µm) (Agilent, Palo Alto, CA, USA). A splitless injector suitable for SPME was used. After extraction, the fiber was removed from the headspace vial and inserted directly into the injection port of the GC. The SPME fiber was thermally desorbed for 2.5 min at 260 °C.
The initial temperature was 40 °C for 5 min, and this was subsequently increased at 4 °C/min using the splitless injection mode for 5 min up to 250 °C. GC-MS detection was performed in complete scanning mode (SCAN) in the 40–350 amu mass range with two scans per second. Electron impact mass spectra were recorded at an ionization voltage of 70 eV and an ion source of 280 °C. The volatile concentrations reported were calculated by dividing the peak area of the compounds of interest by the peak area of the internal standard (normalized area). The relative response factor was considered to be 1. Identification was performed by comparison of the mass spectra with the mass spectra library database Wiley 6.0., and retention times with those of pure standards when they were available.

Chromatographic analysis was carried out in a 6890N Network GC system coupled to MS 5973 Network selective detector (Agilent, Palo Alto, CA, USA). Helium was used as a carrier gas. Separations were accomplished in a DB Wax USN 125-7031 column (30 m × 0.25 mm × 0.25 µm) (Agilent, Palo Alto, CA, USA). A splitless injector suitable for SPME was used. After extraction, the fiber was removed from the headspace vial and manually inserted directly into the injection port of the GC. The SPME fiber was thermally desorbed for 4 min at 260 °C.
The initial temperature was held at 40 °C for 5 min and increased at from 40 °C to 190 °C at 3 °C/min and from 190 °C to 220 °C at 10 °C/min which was held for 5 min using splitless injection mode. GC-MS detection was performed in complete scanning mode (SCAN) in the 40–350 amu mass range with two scans per second. Electron impact mass spectra were recorded at an ionization voltage of 70 eV and ion source of 280 °C. Volatile concentrations reported were calculated by dividing the peak area of the compounds of interest by the peak area of the internal standard (normalized area). The relative response factor was considered to be 1. Tentative Identification was performed by comparison of their mass spectra with those of the mass spectra library database Wiley 6.0., and their retention times with those of pure standards when they were available.