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3 protocols using boric acid

1

Comprehensive Phytochemical Analysis of Plants

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Hydrochloric acid, bromocresol green and methyl red indicators, boric acid, Folin–Ciocalteu’s reagent and sodium carbonate were supplied by Kemika (Zagreb, Croatia). Kjeldahl tablets were purchased from CarlRoth (Karsruhe, Germany). An integrated total dietary fibre assay kit was purchased from Megazyme (Wicklow, Ireland). Gallic acid (>97%), rosmarinic acid (97%), caffeic acid (HPLC standard), cryptochlorogenic (>98%), chlorogenic acid (95%), rutin trihydrate (>97%), luteolin (>98%), apigenin (>97%), (S)-6-Methoxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol was supplied from Panreac (Barcelona, Spain) and sulfuric acid, hexane, ethanol, formic acid and acetonitrile from Carlo Erba (Val de Reuil, France). Acetone was supplied by Gram-mol d.o.o (Zagreb, Croatia). All chemicals used for experimental procedures were of analytical grade or HPLC grade.
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2

Enzyme Characterization for Grape Processing

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Acetonitrile of HPLC grade was purchased from J. T. Baker (Deventer, The Netherlands). Formic acid, glacial acetic acid, and 85 % orthophosphoric acid were obtained from Fluka (Buchs, Switzerland). Acetone, calcium chloride, boric acid and 1 M sodium hydroxide solution were provided from Kemika (Zagreb, Croatia).
The standards used for the identification and quantification purposes were as follows: epigallocatechin, procyanidin B1 and procyanidin B2 (Extrasynthese, Genay Cedex, France); gallic acid, (−)-epicatechin, (+)-catechin and epicatechin-gallate (Sigma-Aldrich, St. Louis, MO, USA).
Lallzyme HC and Lallzyme EX-V isolated from Aspergillus niger were obtained from Lallemand Inc. (Montreal, Canada). The features of these enzyme preparations are given in Table 1.

Commercial names and main activities of enzyme preparations

Enzyme preparationMain activities
Polygalacturonase (PG U g−1)Pectin liase (PL Ug−1)Pectin methylestherase (PME U g−1)Cellulase and hemicellulase
Lallzyme EX-V40001201000+++
Lallzyme HC3500100800
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3

Comprehensive Banana and Beetroot Analysis

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Banana (var. Cavendish, produced by Costanza Organic Bananas, Ecuador) and red beetroot (grown at a local agricultural farm) were purchased in a local store. Hydrochloric acid (37%), bromcresol green and methyl red indicators, boric acid and Folin-Ciocalteu's reagent were supplied from Kemika (Zagreb, Croatia). Kjeldahl tablets were purchased from CarlRoth (Karsruhe, Germany). An integrated total dietary fiber assay kit was purchased from Megazyme (Wicklow, Ireland). Dopamine hydrochloride, (S)-6-Methoxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2 -Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), gallic acid, neutral red and dichlorofluorescein diacetate (DCFDA) were purchased from Sigma-Aldrich (St. Louis, USA). Standards of D-glucose, D-fructose and sucrose were purchased from Fluka (Taufkirchen, Germany). Methanol was supplied from Panreac (Barcelona, Spain) and ethanol (96%), glacial acetic acid (85%) and acetonitrile from Carlo Erba (Val de Reuil, France). All chemicals used for experimental procedures were of analytical grade.
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