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2 protocols using methyltestosterone

1

Procurement of Diverse Chemical Compounds

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Abietic acid, ADD, DHEA, methyltestosterone,
phenanthrene, and progesterone were purchased from Tokyo Chemical
Industry (Tokyo, Japan). DehydroAbietic acid, dibenzothiophene, 7-ethoxycoumarin,
ibuprofen, and testosterone were purchased from Fujifilm Wako Pure
Chemical (Osaka, Japan). Pyrene was purchased from Sigma-Aldrich Japan
(Tokyo, Japan). Diclofenac was purchased from Combi-Blocks USA (San
Diego, CA, USA). Ferruginol was kindly provided by Dr. H. Suhara (Miyazaki
Prefectural Wood Utilization Research Center, Japan). Yeast nitrogen
base without amino acids was purchased from Formedium (Hunstanton,
UK). Dropout supplements (DOS) were purchased from TaKaRa Bio USA
(Mountain View, CA, USA). Custom-synthesized oligonucleotide primers
were obtained from Sigma-Aldrich Japan. All other chemicals were of
reagent grade. Deionized water was obtained using a Barnstead Smart2Pure
System (Thermo Fisher Scientific, Waltham, MA, USA).
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2

Bisphenol A Bioactivation Assay

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Bisphenol A (>99%; CAS 80-05-7), BPA sulfate, sodium salt (95%, CAS 847696-37-1), BPF (98%; CAS 620-92-8), BPS (98%; CAS 80-09-1), dithiothreitol (98%; CAS 3483-12-3), resazurin, sodium salt (80%; CAS 62758-13-8), MgCl 2 (≥98%; CAS 7786-30-3), 3 ′ -phosphoadenosine-5 ′phosphosulfate, and dimethyl sulfoxide were all from Sigma-Aldrich (St. Louis, MO, USA). 17-β-Estradiol (E2; ≥98%; CAS 50-28-2), 17-α-estradiol (≥98%; CAS 57-91-0), corticosterone (≥98.5%; CAS 50-22-6), and kanamycin solution from Streptomyces kanamyceticus (10 mg/mL; CAS 25389-94-0) were from Sigma-Aldrich (Germany). Pooled human liver cytosol (total protein concentration, 20 mg/mL) was from Thermo Fisher Scientific (MA, USA). Methyltestosterone (>98%; CAS 58-18-4) was from Tokyo Chemical Industry (TCI Europe, Belgium). Methanol and acetonitrile were LC-MS grade.
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