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Chloroform and lc ms grade acetonitrile

Manufactured by Thermo Fisher Scientific

Chloroform is a colorless, volatile liquid commonly used as a solvent in various laboratory applications. LC/MS grade acetonitrile is a high-purity solvent used in liquid chromatography-mass spectrometry (LC/MS) analysis.

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2 protocols using chloroform and lc ms grade acetonitrile

1

Preparation and Characterization of Liposomal Formulations

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Lecithin (Phospholipon 90 G) was obtained as a gift from Lipoid (LudwiGSHafen, Germany). Cholesterol was supplied by MP Biomedicals (Solon, Ohio), dimethyldioctadecylammonium bromide (DDAB) and GSSG were obtained from Applichem (Darmstadt, Germany). GSH was obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO). Chloroform and LC/MS grade acetonitrile, water and formic acid were obtained from Fisher Scientific (Fair Lawn, NJ). 5-Sulfosalicylic acid was purchased from J.T. Baker Chemical Co. (Phillipsburg, NJ). RPMI 1640 growth medium, penicillin/streptomycin, phosphate buffered saline (PBS), and trypsin were obtained from Mediatech (Herndon, VA). Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Lawrenceville, GA). NCI-H226 cells (human non-small cell lung carcinoma) were obtained from the National Cancer Institute.
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2

Formulation of Phospholipid-Based Delivery

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Lecithin (Phospholipon 90G) was obtained as a gift from Lipoid (LudwiGSHafen, Germany). Cholesterol was supplied by MP Biomedicals (Solon, Ohio), dimethyldioctadecylammonium bromide (DDAB) and GSSG were obtained from Applichem (Darmstadt, Germany). GSH was obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO). Chloroform and LC/MS grade acetonitrile, water and formic acid were obtained from Fisher Scientific (Fair Lawn, NJ). 5-Sulfosalicylic acid was purchased from J.T. Baker Chemical Co. (Phillipsburg, NJ). RPMI 1640 growth medium, penicillin/streptomycin, phosphate buffered saline (PBS), and trypsin were obtained from Mediatech (Herndon, VA). Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Lawrenceville, GA). NCI-H226 cells (human non-small cell lung carcinoma) were obtained from the National Cancer Institute.
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