Hypochlorous sodium solution

Manufactured by Merck Group

Hypochlorous sodium solution is a stable, aqueous solution containing hypochlorous acid and sodium hypochlorite. It serves as a disinfectant and oxidizing agent for various laboratory applications.

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2 protocols using hypochlorous sodium solution

Insulin Signaling in 3T3-L1 Adipocytes

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Mouse 3T3-L1 preadipocytes were obtained from American Type Culture Collection (Manassas, VA). 3T3-L1 preadipocyte medium, 3T3-L1 adipocyte differentiation medium, and 3T3-L1 adipocyte maintenance medium were obtained from Zen-Bio, Inc. (Research Triangle Park, NC). [1, 2-³H] 2-deoxy-D-glucose was purchased from Perkin Elmer Life Sciences (Waltham, MA). Hypochlorous sodium solution, human insulin, Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME), SP600125, and antibody against phospho-IRS-1 (Tyr612) were purchased from Sigma-Aldrich (St. Louis, MO). Protein A/G-agarose, RIPA lysis buffer, PS-1145, IKKβ siRNA, JNK2 siRNA, PKCθ siRNA, control siRNA, and antibodies against β-actin, GAPDH and Na⁺/K⁺ ATPase were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies against phospho-IRS-1 (Ser307), IRS-1, phospho-Akt (Ser473), phospho-Akt (Thr308), Akt, phospho-GSK3β (Ser9), GSK3β, phospho-IKKα/β (Ser176/180), IKKα, IKKβ, phospho-SAPK/JNK (Thr183/Tyr185), JNK, phospho-PKCθ (Thr538), PKCθ, GLUT4, IκBα, and horseradish peroxidase-linked secondary antibodies (Beverly, MA). ONOO⁻ was obtained from Calbiochem (Billerica, MA). All other chemicals were of the highest commercial grade available.

Zhou J., Wang Q., Ding Y, & Zou M.H. (2015). Hypochlorous acid via peroxynitrite activates protein kinase Cθ and insulin resistance in adipocytes. Journal of Molecular Endocrinology, 54(1), 25-37.

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Insulin Resistance in 3T3-L1 Adipocytes

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Mouse 3T3-L1 preadipocytes were obtained from American Type Culture Collection (Manassas, VA, USA). 3T3-L1 preadipocyte medium, 3T3-L1 adipocyte differentiation medium, and 3T3-L1 adipocyte maintenance medium were obtained from Zen-Bio, Inc. (Research Triangle Park, NC, USA). [1,2-³H] 2-deoxy-d-glucose was purchased from Perkin Elmer Life Sciences (Waltham, MA, USA). Hypochlorous sodium solution, human insulin, Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME), SP600125, and antibody against phospho-IRS1 (Tyr612) were purchased from Sigma–Aldrich. Protein A/G-agarose, RIPA lysis buffer, PS-1145, IKKβ siRNA, JNK2 siRNA, PKCθ siRNA, control siRNA, and antibodies against β-actin, GAPDH, and Na⁺/K⁺ ATPase were obtained from Santa Cruz Biotechnology, Inc. Antibodies against phospho-IRS1 (Ser307), IRS1, phospho-Akt (Ser473), phospho-Akt (Thr308), Akt, phospho-GSK3β (Ser9), GSK3β, phospho-IKKα/β (Ser176/180), IKKα, IKKβ, phospho-SAPK/JNK (Thr183/Tyr185), JNK, phospho-PKCθ (Thr538), PKCθ, GLUT4, IκBα, and HRP-linked secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). ONOO⁻ was obtained from Calbiochem (Billerica, MA, USA). All other chemicals were of the highest commercial grade available.

Zhou J., Wang Q., Ding Y, & Zou M.H. (2015). Hypochlorous acid via peroxynitrite activates protein kinase Cθ and insulin resistance in adipocytes. Journal of Molecular Endocrinology, 54(1), 25-37.

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