Amplification data were handled in a Bio-Rad CFX Maestro v 3.0 (Bio-Rad, Hercules, CA, USA), with Ct determination mode set to a single threshold and the baseline decided by baseline subtracted curve fit. Unknown cell concentrations were derived directly from the standard calibration curve by a Bio-Rad CFX Maestro v 3.0 (Bio-Rad, Hercules, CA, USA). Raw data were extracted to Microsoft Excel Professional Plus 2010 (Microsoft, Redmond, WA, USA) and OriginPro 2019b (Originlab Co., Northampton, MA, USA), where they were inspected manually.
Hu J., Guo R., Lu D., Dai X., Zhu Y., Park B.S, & Wang P. (2022). Detection and Quantification of the Harmful Dinoflagellate Margalefidinium polykrikoides (East Asian Ribotype) in the Coastal Waters of China. Toxins, 14(2), 95.
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