Total blood cell counts were performed in fresh human EDTA blood samples using a Beckman Coulter Counter (Beckman Coulter Inc. USA). Plasma UCB and haem concentrations were quantified using HPLC and a photodiode array detector (Waters, Australia) as previously described [15 (link)]. A C18 reverse-phase HPLC guard and analytical column (4.6 × 150 mm, 3 μM; Phenomenex, Australia) was perfused at 0.7 mL/min using methanolic 0.1 M di-n-octylamine acetate (methanol:H2O 95:5 v/v) mobile phase. The extracted samples were injected with a run time of 18 min and were analysed in duplicate. Haem (λmax 400 nm) and UCB (λmax 450 nm) eluted at 8 and 13 mins, respectively. Haemin and UCB (Frontier Scientific, Logan UTA, USA) at a concentration of 0-100 μM were used for external standards.
C18 reverse phase hplc guard and analytical column
Manufactured by Phenomenex
Sourced in Australia
The C18 reverse-phase HPLC guard and analytical column is a laboratory instrument used for high-performance liquid chromatography (HPLC) analysis. The column is packed with a stationary phase of octadecyl-modified silica particles, which allows for the separation and analysis of a wide range of organic compounds. The guard column is designed to protect the analytical column from contaminants, extending its lifespan and performance.
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Lab products found in correlation
2 protocols using c18 reverse phase hplc guard and analytical column
1
Quantifying Blood Cell Counts and Bilirubin Levels
2
Quantifying Blood Cell Counts and Bilirubin Levels
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Total blood cell counts were performed in fresh human EDTA blood samples using a Beckman Coulter Counter (Beckman Coulter Inc. USA). Plasma UCB and haem concentrations were quantified using HPLC and a photodiode array detector (Waters, Australia) as previously described [15 (link)]. A C18 reverse-phase HPLC guard and analytical column (4.6 × 150 mm, 3 μM; Phenomenex, Australia) was perfused at 0.7 mL/min using methanolic 0.1 M di-n-octylamine acetate (methanol:H2O 95:5 v/v) mobile phase. The extracted samples were injected with a run time of 18 min and were analysed in duplicate. Haem (λmax 400 nm) and UCB (λmax 450 nm) eluted at 8 and 13 mins, respectively. Haemin and UCB (Frontier Scientific, Logan UTA, USA) at a concentration of 0-100 μM were used for external standards.
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