Primary antibodies were used in the following dilutions: anti-FLAG M2 mouse monoclonal 1:100 (F1804, Sigma), α-tubulin mouse monoclonal 1:1,000 (T6199, Sigma), and laminin rabbit polyclonal 1:100 (L9393, Sigma). Secondary antibodies and phalloidin were used in the following dilutions: Alexa 488 rabbit 1:1,000 (A11008, Invitrogen, Molecular Probes), Alexa 594 mouse 1:1,000 (A21125, Invitrogen, Molecular Probes), and Phalloidin 635 1:200 (A34054, Thermo Fisher Scientific). Tissue sections were subsequently analyzed using the confocal microscope Leica SPE.
A34054
Manufactured by Thermo Fisher Scientific
The A34054 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for use in scientific and research applications. The core function of this product is to provide a reliable and consistent performance for the intended tasks. No further details about the intended use or specific applications are available.
Automatically generated - may contain errors
2 protocols using a34054
1
Immunofluorescence Labeling of Proteins
2
Immunofluorescence Labeling of Proteins
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