Anti c myc hrp 11814150001

The TRIM45 expression plasmid was constructed using the GATEWAY System (Life Technologies, Carlsbad, CA, USA). The HA-tagged p53 expression plasmid and the p53 domain deletion mutants were kindly provided by Dr. Tiebang Kang (Sun Yat-sen University, Guangzhou, China). All mutants were generated using standard molecular techniques. The anti-TRIM45 antibody (NBP1-53109) was purchased from Novus (Littleton, CO, USA) and anti-p53 (sc-126) was obtained from Santa Cruz (Santa Cruz, CA, USA). Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592) and anti-β-actin (A1978) were purchased from Sigma (St. Louis, MO, USA). HRP-anti-hemagglutinin (12013819001) and anti-c-Myc-HRP (11814150001) were purchased from Roche Applied Science (Mannheim, Germany). The anti-caspase-3 (25546-1-AP) was purchased from Proteintech (Wuhan, Hubei, P.R.C). The anti-MDM2 (YT5186) was purchased from ImmunoWay (Newark, DE, USA). The anti-K63-linked ubiquitin antibody (05-1308) was obtained from Millipore (Billerica, MA, USA). The anti-phospho-Akt (no. 4060), anti-Akt (no. 4691), anti-phospho-JNK (no. 9251), anti-JNK (no. 9252), anti-phospho-ERK (no. 9101), anti-ERK (no. 9102), anti-phospho-p38 (no. 9211), anti-p38 (no. 9212), anti-Puma (no. 4976), and anti-K48-linked ubiquitin (no. 4289) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA).

Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592) and anti-β-actin (A1978) were purchased from Sigma; HRP-anti-hemagglutinin (12013819001), anti-c-Myc-HRP (11814150001) were purchased from Roche Applied Science; anti-phospho-IKKα/β (No.2697S), anti-phospho-JNK (No.9251), anti-JNK (No.9252), anti-phospho-ERK (No.9101), anti-ERK (No.9102), anti-phospho-p38 (No.9211), anti-p38 (No.9212), anti-p65 (No.6956), anti-IκBα (No.4814) and anti-phospho-IkBα (Ser32/36) (No. 9246) were from Cell Signaling Technology; anti-NEMO (sc-8300), anti-USP18(sc-98431) were purchased from Santa Cruz Biotechnology; anti-IKKβ were purchased from Millipore; CF568 Goat anti-mouse IgG (H+L) (20101-1) were purchased from Biotium; anti-linear polyubiquitin-specific monoclonal antibody (AB130) were purchased from LifeSensors.
Recombinant human TNF-α was purchased from PeproTech; Lipopolysaccharides (LPS) (L4391-1 MG) were purchased from Sigma. Pam3CSK4 and CL097 were purchased from InvivoGen.