Ion exchange high performance liquid chromatography method

Manufactured by Bio-Rad

The ion-exchange high-performance liquid chromatography (HPLC) method is a laboratory technique used for the separation and purification of charged molecules, such as proteins, peptides, and nucleic acids. This method utilizes a stationary phase containing charged functional groups that interact with the charged analytes, allowing for their separation based on their charge and size characteristics. The core function of this technique is to provide a reliable and efficient means of isolating and analyzing charged biomolecules in a complex mixture.

Automatically generated - may contain errors

Lab products found in correlation

Hexokinase method, by Roche (1 mentions) Glucose oxidase method, by Beckman Coulter (1 mentions) Immulite, by Siemens (1 mentions)

2 protocols using ion exchange high performance liquid chromatography method

Blood Sampling and Biomarker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Upon participant arrival at the laboratory (V₁), a 20‐gauge polyethylene catheter was inserted into a forearm vein for blood sampling. Blood samples were drawn at rest after an overnight fasting. Fasting blood glucose was measured using the hexokinase method (Roche Diagnosis, Indianapolis, Indiana). Glycated hemoglobin was assayed using the ion‐exchange high‐performance liquid chromatography method (Bio‐Rad, Hercules, California). Serum cholesterol, triglycerides and high‐density lipoprotein‐cholesterol were analyzed as previously described (Poirier et al. 2000, 2001). Low‐density lipoprotein‐cholesterol was calculated using Friedewald's formula (Friedewald et al. 1972). The cholesterol/high‐density lipoprotein ratio was also calculated.

Caron J., duManoir G.R., Labrecque L., Chouinard A., Ferland A., Poirier P., Legault S, & Brassard P. (2017). Impact of type 2 diabetes on cardiorespiratory function and exercise performance. Physiological Reports, 5(4), e13145.

+ Open protocol

+ Expand

Glucose, Insulin, and HbA1c Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma glucose was measured by the glucose oxidase method (Beckman Instruments, Fullerton, CA) and plasma insulin by a chemiluminescent assay using commercial kits (Immulite, Siemens Ltd., Llanberis, Gwynedd, UK). An ion-exchange high-performance liquid chromatography method (Bio-Rad, Hercules, CA) was used to measure HbA1c. The within-run and between-run coefficients of variation for the HbA1c assay were 0.82% and 0.51%, respectively.

Ebenibo S., Edeoga C., Wan J, & Dagogo-Jack S. (2014). Glucoregulatory Function among African Americans and European Americans with Normal or Pre-diabetic Hemoglobin A1c Levels. Metabolism: clinical and experimental, 63(6), 767-772.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!