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Tsc2 shrna

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The TSC2 shRNA is a small hairpin RNA (shRNA) product that targets the TSC2 gene. The TSC2 gene encodes the tuberin protein, which is a component of the TSC1-TSC2 complex that acts as a negative regulator of the mTOR signaling pathway. The TSC2 shRNA can be used to knockdown the expression of the TSC2 gene, which may be useful for studying the role of the TSC1-TSC2 complex in cellular processes.

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Lab products found in correlation

Scramble shrna, by Addgene (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions)

3 protocols using tsc2 shrna

1

Genetic Manipulation of Organoids

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RPTOR shRNA, TSC2 shRNA (Addgene) and CAG-GFP plasmids were electroporated at 1 µg/ul using a BTX electroporator set at 5V for 5 milliseconds for three pulses. Organoids were bathed in 200 µl media containing plasmid for 10mins. Then, an electric current was passed through the cuvette. Organoids were maintained for 4–10 days in culture prior to processing.
Andrews M.G., Subramanian L, & Kriegstein A.R. (2020). mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex. eLife, 9, e58737.
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2

siRNA Knockdown and Stable Cell Line

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Cells were transfected with 50 nM siRNA twice on two consecutive days by Lipofectamine RNAiMAX reagents (Invitrogen, Grand Island, NY, USA), and assayed 48 h after the first transfection. The sources and sequences of siRNA could be found in the Supplementary information. To generate a stable TSC2 knockdown cell line, plasmids encoding scramble shRNA (Addgene, Cambridge, MA, USA, plasmid 1864) or TSC2 shRNA (Addgene, plasmid 15478) were used to generate lentivirus. 420 cells were infected with the virus and selected with 2 μg/mL puromycin. Knockdown efficiency was examined by immunoblotting.
Yang C.S., Matsuura K., Huang N.J., Robeson A.C., Huang B., Zhang L, & Kornbluth S. (2014). Fatty Acid Synthase inhibition engages a novel caspase-2 regulatory mechanism to induce ovarian cancer cell death. Oncogene, 34(25), 3264-3272.
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3

siRNA Knockdown and Stable Cell Line

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Cells were transfected with 50 nM siRNA twice on two consecutive days by Lipofectamine RNAiMAX reagents (Invitrogen, Grand Island, NY, USA), and assayed 48 h after the first transfection. The sources and sequences of siRNA could be found in the Supplementary information. To generate a stable TSC2 knockdown cell line, plasmids encoding scramble shRNA (Addgene, Cambridge, MA, USA, plasmid 1864) or TSC2 shRNA (Addgene, plasmid 15478) were used to generate lentivirus. 420 cells were infected with the virus and selected with 2 μg/mL puromycin. Knockdown efficiency was examined by immunoblotting.
Yang C.S., Matsuura K., Huang N.J., Robeson A.C., Huang B., Zhang L, & Kornbluth S. (2014). Fatty Acid Synthase inhibition engages a novel caspase-2 regulatory mechanism to induce ovarian cancer cell death. Oncogene, 34(25), 3264-3272.
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