Biotin-labeled miR-2861 was purchased from Genewiz (Jiangsu, China). Biotin-labeled miR-2861 (3 μg) was incubated with streptavidin-coated beads (434341; Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C overnight. Then, streptavidin-coated beads were incubated with SV40-MES-13 cell lysate at 4 °C for 12 h. RNAs bound by miR-2861 were purified with Trizol reagent (Takara, Shiga, Japan) and assessed through qRT-PCR.
Streptavidin coated beads 434341
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
Streptavidin-coated beads (434341) are a type of lab equipment used for various biomedical applications. They consist of magnetic beads coated with the protein streptavidin, which has a high affinity for the small molecule biotin. These beads can be used to capture and isolate biotinylated molecules, such as proteins, nucleic acids, or cells, from complex samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using streptavidin coated beads 434341
1
Biotin-Labeled miR-2861 Pulldown Assay
2
Biotin-miR-2861 Affinity Purification
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Biotin-labeled miR-2861 was purchased from Genewiz (Jiangsu, China).
Biotin-labelled miR-2861 (3 μg) was incubated with streptavidin-coated beads (434341; Thermofisher Scientific) at 4 °C overnight. Then, streptavidin-coated beads were incubated with mesangial cell lysate at 4 °C for 12 h. RNAs bound by miR-2861 were purified with Trizol reagent (Takara, Shiga, Japan), and detected through qRT-PCR assay.
Biotin-labelled miR-2861 (3 μg) was incubated with streptavidin-coated beads (434341; Thermofisher Scientific) at 4 °C overnight. Then, streptavidin-coated beads were incubated with mesangial cell lysate at 4 °C for 12 h. RNAs bound by miR-2861 were purified with Trizol reagent (Takara, Shiga, Japan), and detected through qRT-PCR assay.
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