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Chi3l1 chi3l1

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CHI3L1/Chi3l1 is a protein that functions as a chitin-binding lectin. It is involved in various biological processes, including inflammation, tissue remodeling, and cell signaling. This protein is commonly used in research applications to study its role in these processes.

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Lab products found in correlation

Smgm 2, by Lonza (2 mentions) Ebm 2, by Lonza (2 mentions) Interleukin 4 (il 4), by R&D Systems (2 mentions) Interleukin 6 (il 6), by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

CHI3L1 Chi3l1 ELISA kit Mouse recombinant CHI3L1 protein

2 protocols using chi3l1 chi3l1

1

Aortic Cell Cytokine Stimulation

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Human aortic smooth muscle cells (AoSMC) and human aortic endothelial cells (AoEC) were propagated in growth media [SmGM-2 (for AoSMC) and EBM-2 (for AoEC); Lonza] with 5% fetal bovine serum (FBS) per standard protocols (Lonza; passage #4-5). AoSMC were incubated in basal medium [SmBM] for 48 hours prior to treatment/transfection, and other lines were serum-starved overnight prior to treatment/transfection.RAW 264.7 cells (ATCC) were propagated in DMEM + 10% FBS. Cells were treated with human or murine recombinant IL-6 (20ng/ml) (Cell Signaling), IL-4 (5 or 30ng/ml), and/or CHI3L1/Chi3l1 (30 or 300ng/ml) (R&D Biosystems) for 12h or 24h. Wells were harvested for RNA analysis at approximately 90% confluence.
Maegdefessel L., Spin J.M., Raaz U., Eken S.M., Toh R., Azuma J., Adam M., Nagakami F., Heymann H.M., Chernugobova E., Jin H., Roy J., Hultgren R., Caidahl K., Schrepfer S., Hamsten A., Eriksson P., McConnell M.V., Dalman R.L, & Tsao P.S. (2014). miR-24 limits aortic vascular inflammation and murine abdominal aneurysm development. Nature Communications, 5, 5214.
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2

Cytokine Stimulation of Human Vascular Cells

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Human aortic SMC (hASMCs) and human AoECs were propagated in growth media (SmGM-2 (for hASMC) and EBM-2 (for AoEC); Lonza) with 5% fetal bovine serum (FBS) per standard protocols (Lonza; passage no. 4–5). hASMC were incubated in basal medium (SmBM) for 48 h before treatment/transfection, and other lines were serum-starved overnight before treatment/transfection. RAW 264.7 cells (ATCC) were propagated in DMEM+10% FBS. Cells were treated with human or murine recombinant IL-6 (20 ng ml−1; Cell Signaling), IL-4 (5 or 30 ng ml−1) and/or CHI3L1/Chi3l1 (30 or 300 ng ml−1; R&D Biosystems) for 12 or 24 h. Wells were harvested for RNA analysis at ~90% confluence.
Maegdefessel L., Spin J.M., Raaz U., Eken S.M., Toh R., Azuma J., Adam M., Nagakami F., Heymann H.M., Chernugobova E., Jin H., Roy J., Hultgren R., Caidahl K., Schrepfer S., Hamsten A., Eriksson P., McConnell M.V., Dalman R.L, & Tsao P.S. (2014). miR-24 limits aortic vascular inflammation and murine abdominal aneurysm development. Nature Communications, 5, 5214.
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