Ampicillin (1 mg/ml) (A8180, Solarbio, China), streptomycin (1 mg/ml) (S8290, Solarbio, China), vancomycin (0.25 mg/ml) (V8050, Solarbio, China), and neomycin (1 mg/ml) (N8090, Solarbio, China) were added to the milk and fed for 5 days to eliminate as much as possible the gut microbial background of the piglets, followed by FMT or feeding of bacteria or metabolites. Feces were collected on day 8 from the uninfected group of Min pigs and Landrace pigs and treated as described previously. Briefly, fecal samples were placed in a sterile N2 incubator and mixed thoroughly. Then, cell filters were inserted to collect the bacteria and centrifuged at 600×g for 10 min. The samples were then added to 10% glycerol and stored at −80 °C [23 (link)]. Prior to FMT, PBS was used instead of glycerol, and the number of viable bacteria was subsequently counted by methylene blue staining. Two milliliters of the bacterial solution were mixed into the milk and fed to the piglets for three consecutive days, and the PEDV challenge occurred on day 0 by gavage.
N8090
Manufactured by Solarbio
Sourced in China
The N8090 is a bench-top model centrifuge designed for general laboratory use. It features variable speed control with a maximum speed of 6,000 RPM and can accommodate a maximum sample volume of 100 mL. The centrifuge is equipped with a sturdy metal housing and a digital display for monitoring speed and time.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using n8090
1
Gut Microbiome Restoration in Piglets
2
Evaluating Selectivity of Pepper-based SAM Sensors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To test the selectivity of the Pepper-based SAM sensors, Pepper-based SAM sensor RNA (1 μM) was mixed with HBC (10 μM) in the presence of the target (0.1 mM) or its analogs in the buffer. The samples were incubated at 37°C for 1 h. The fluorescence signal of each sample was measured at 37°C using a spectrofluorometer with 505 nm excitation, 545 nm emission, 5 nm slit widths and 0.1 s integration time. Analogs of SAM include SAH (Aladdin S139501), adenosine (Coolaber CA1241) or methionine (Coolaber CM7211). Analogs of tetracycline include doxycycline (Solarbio ID0670), neomycin (Solarbio N8090), tobramycin (Solarbio T8810), gentamycin (Solarbio G8170), ampicillin (Solarbio A1170) and kanamycin (Solarbio K1030). Analogs of guanine including guanosine (MACKLIN G810366) and adenine (BBI A600013-0025) were used in guanine sensor selectivity measurement. Bovine serum albumin (BSA; Cusabio NP009501B), lysozyme (Cusabio NP004301C) and ovalbumin (Cusabio NP004201C) was used in streptavidin sensor selectivity measurement.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!