Anti mouse cd4 gk1

Manufactured by BD

Sourced in France

The Anti-mouse CD4 (GK1.5) is a monoclonal antibody that binds to the CD4 receptor on the surface of mouse T cells. CD4 is a cell surface glycoprotein that is primarily expressed on a subset of T cells called T helper cells. The antibody can be used in various laboratory applications, such as flow cytometry, immunohistochemistry, and cell depletion experiments.

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Lab products found in correlation

Lsrfortessa flow cytometer, by BD (1 mentions) Anti mouse ifn γ xmg1, by Thermo Fisher Scientific (1 mentions) Anti mouse ctla 4 uc10 4b9, by Thermo Fisher Scientific (1 mentions) Anti mouse cd11c n418, by Thermo Fisher Scientific (1 mentions) Anti mouse foxp3 fjk 16s, by Thermo Fisher Scientific (1 mentions) Fixable viability dye efluor 780, by BD (1 mentions) Anti mouse cd8 53 6, by Thermo Fisher Scientific (1 mentions) Facscanto 2 cytometer, by BD (1 mentions) Cd25 pc61, by BD (1 mentions) Cd19 1d3, by BD (1 mentions) Cd8 53 6, by BD (1 mentions) Cd3 145 2c11, by BD (1 mentions) Il 10 jes5 16e3, by BD (1 mentions)

Close spelling variants of this product

Anti-CD4 (255 citations) Anti-mouse CD4 (31 citations) CD4 (GK1.5, (29 citations) Anti-CD4 (GK1.5, (9 citations) Anti-mouse CD4 (clone GK1.5, (4 citations) Mouse anti (2 citations) APC-H7 anti-mouse CD4 (GK1.5, (2 citations) Mouse anti-CD4+ FITC (clone GK1.5) (2 citations)

2 protocols using anti mouse cd4 gk1

Comprehensive Immunophenotyping of Immune Cells

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Fluorochrome-conjugated monoclonal/polyclonal antibodies used in our studies were anti-mouse CD4 (GK1.5, BD Biosciences), anti-mouse CD25 (PC61.5l, eBioscience), anti-mouse Foxp3 (FJK-16s, eBioscience), anti-mouse CD8 (53-6.7, eBioscience), anti-human CD19 (H1B19, eBioscience), anti-human EGFR (AY13, BioLegend), anti-mouse CD45.1 (A20, eBioscience), anti-mouse CTLA-4 (UC10-4B9, eBioscience), anti-mouse neuropilin 1 (eDS304M, eBioscience), anti-mouse Lag-3 (C9B7W, eBioscience), anti-mouse CD11c (N418, eBioscience), anti-mouse IFN-γ (XMG1.2, eBioscience), anti-mouse TNF-α (MP6-XT22, eBioscience), anti-mouse Fas (SA367H8, BioLegend), anti-mouse FasL (MFL3, eBioscience), anti-mouse perforin (eBioMAK-D, eBioscience), anti-mouse GzB (NGZB, eBioscience), anti-mouse GzA (3G8.5, BioLegend), anti-mouse CD107α (1D4B, eBioscience), anti-mouse CD71 (R17217, eBioscience), anti-mouse CPT1a (8F6AE9, Abcam), anti-mouse Glut1 (ER3915, Abcam), and Fixable viability dye eFluor 780 (BD Biosciences). Intracellular staining was performed using the fixation/permeabilization concentrate (catalog 5123-43) and diluent (catalog 5223-56) buffer solutions and IC buffer (catalog 8333-56), according to the eBioscience Foxp3 staining kit. Stained cells were analyzed on an LSR Fortessa flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).

Bolivar-Wagers S., Loschi M.L., Jin S., Thangavelu G., Larson J.H., McDonald-Hyman C.S., Aguilar E.G., Saha A., Koehn B.H., Hefazi M., Osborn M.J., Jensen M.C., Wagner J.E., Pennell C.A, & Blazar B.R. (2022). Murine CAR19 Tregs suppress acute graft-versus-host disease and maintain graft-versus-tumor responses. JCI Insight, 7(17), e160674.

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Multicolor Flow Cytometry of Immune Cells

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Antimouse CD4 (GK1.5, 1/300), CD8 (53-6.7, 1/300), CD3 (145 2C11, 1/200), CD19 (1D3, 1/400), CD25 (PC61, 1/200) and IL-10 (JES5-16E3, 1/50) and anti-human CD3 antibodies were from BD Biosciences (Le Pont de Claix, France). Antimouse FOXP3 antibodies (FJK-16S, 1/200) were from eBioscience (Life Technologies, Saint-Aubin, France). Detection of autoantigen-specific CD8 + T cells was performed using allophycocyanin (APC)-labelled class I MHC (H-2K d ) tetramers carrying the proinsulin (PI)-B 15-23 or the isletspecific glucose-6-phosphatase catalytic subunit related protein (IGRP) 206-214 peptides (provided by the NIH Tetramer Core Facility, Atlanta, GA, USA). Briefly, cells were stained with APC-labelled tetramers (0.5 μl/50 μl PBS per well) at 4°C for 1 h. After two washes, cell surface antibodies were added for 20 min of incubation. Cells were analysed on a FACS CANTO II cytometer (BD Biosciences) using the FlowJo software (FlowJo, Ashland, OR, USA).

Besançon A., Goncalves T., Valette F., Dahllöf M.S., Mandrup-Poulsen T., Chatenoud L, & You S. (2018). Oral histone deacetylase inhibitor synergises with T cell targeted immunotherapy to preserve beta cell metabolic function and induce stable remission of new-onset autoimmune diabetes in NOD mice. Diabetologia, 61(2).

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