Rat α ha antibody

Manufactured by Roche

The Rat α-HA antibody is a laboratory reagent used to detect and identify the HA (hemagglutinin) tag in recombinant proteins expressed in various cell systems, including rat cells. This antibody is a useful tool for researchers studying protein expression, localization, and purification.

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Lab products found in correlation

Ecl detecting reagent, by Thermo Fisher Scientific (1 mentions) Rabbit α gfp, by Abcam (1 mentions) Protein a agarose bead, by Thermo Fisher Scientific (1 mentions) Chemidoc, by Bio-Rad (1 mentions) Goat α mouse, by Merck Group (1 mentions) Goat α rabbit, by Merck Group (1 mentions) Mouse α sag1, by Thermo Fisher Scientific (1 mentions) Tris acetate polyacrylamide gradient gel, by Thermo Fisher Scientific (1 mentions) Mouse α myc, by Cell Signaling Technology (1 mentions)

2 protocols using rat α ha antibody

Immunoblot Analysis of GI, ZTL, and TOC1

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Total proteins were isolated from the samples using protein extraction buffer containing protease and proteasome inhibitors and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) as described previously (13 (link)). Immunoblot analysis was carried out using rat α-HA antibody (1:2,000; Roche) to detect GI-HA, rabbit α-ZTL antibody (1:500) to detect ZTL (3 (link)), and rabbit α-GFP (1:3,000; Abcam) to detect the TOC1 minigene (53 (link)) and SOS1-GFP. To detect native GI protein in sos1 alleles, soluble proteins were extracted from the samples using urea/SDS buffer, and immunoblot analysis was performed with rabbit α-GI antibody (54 ). Proteins were detected based on chemiluminescence using ECL-detecting reagent (Thermo Fisher Scientific) and Chemi-Doc (Bio-Rad). For Co-IP assays, total protein extracts isolated from N. benthamiana coexpressing SOS1-GFP and GI-HA were prepared and incubated with Protein A-agarose beads (Invitrogen) to capture α-GFP (for SOS1-GFP, Thermo Fisher Scientific). The immunoprecipitated proteins were separated by SDS-PAGE and detected as described above.

Cha J.Y., Kim J., Jeong S.Y., Shin G.I., Ji M.G., Hwang J.W., Khaleda L., Liao X., Ahn G., Park H.J., Kim D.Y., Pardo J.M., Lee S.Y., Yun D.J., Somers D.E, & Kim W.Y. (2022). The Na+/H+ antiporter SALT OVERLY SENSITIVE 1 regulates salt compensation of circadian rhythms by stabilizing GIGANTEA in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 119(33), e2207275119.

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Western Blot Analysis of Parasite Proteins

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Confluent HFFs infected with tachyzoites were syringe-lysed 24 hrs postinfection and filtered, followed by centrifugation. Parasite pellets were resuspended in NuPAGE lysis buffer and boiled for 5 min. Eighty µg of parasite lysates were separated on a 4-12% Tris-acetate polyacrylamide gradient gel (Invitrogen) and transferred onto PVDF membranes. Western blotting was performed with the designated antibodies and developed with SuperSignal West Femto Sensitivity Substrate (Pierce).
Primary antibody dilutions were used as follows: rat α-HA antibody (Roche) 1:2,000; mouse α-MYC (Cell Signaling) 1:5,000; rabbit α-MYC (Thermo) 1:5,000; rabbit α-H3 (Sigma) 1:5,000; rabbit eIF2α 1:20,000 (46) , mouse α-Sag1 (Thermo Fisher)
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted June 12, 2020. ; https://doi.org/10.1101/2020.06.09.143586 doi: bioRxiv preprint 18 1:5,000. Secondary horseradish peroxidase (HRP)-conjugated antibody dilutions were used as follows: goat α-rat (GE Healthcare) 1:2,000; goat α-mouse (Sigma) 1:5,000; goat α-rabbit (Sigma) 1:5,000.

Srivastava S., White M.W., & Sullivan W.J. (2020). Toxoplasma gondii AP2XII-2 contributes to proper progression through S-phase of the cell cycle.

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