Rtespa 150 probes

Atomic force microscopy (AFM) imaging (Bruker Icon 3 equipped with Bruker RTESPA-150 probes) in tapping mode was performed on samples of worms (0.01 mg/mL) and amyloid fibrils (0.1 mg/mL) on freshly cleaved mica at a resolution of 1024x1024 lines and at a scan rate of 0.5 Hz. Images were flattened with the Bruker Nanoscope software.

The complexation of the different amyloid fractions with the CNCs in the nanometer range was studied by AFM imaging in tapping mode using a Bruker Icon 3 AFM equipped with Bruker RTESPA-150 probes. The samples were diluted by a factor of 10, using 10 mM HEPES buffer at pH 7 (pure CNCs were diluted to 0.01 mg/mL). After vortexing, 100 lL were dropped onto freshly cleaved mica, adsorbed for 1 min and then rinsed off with 1 mL MilliQ water. For the samples with positive zeta potentials, negatively charged unmodified mica was used, whereas for samples with negative zeta potentials, the mica was modified with APTES to make it positively charged. The images were acquired at a scan rate of 0.5 Hz and a resolution of 1024x1024 lines. The raw data was flattened and processed with the Bruker Nanoscope software.