The detection primers for the 8 SNPs were designed using the MassARRAY Assay Design 3.0 software (Sequenom). Approximately 15-20 ng of genomic DNA for each sample was used to genotype. Eight SNPs were genotyped by using the Sequenom MassArray iPLEX1 system (the Key Laboratory of Dermatology, Ministry of Education, China). The DNA samples were ampli ed by multiplex PCR reactions, and then the PCR products were used for locus-speci c singlebase extension reactions. The resulting products were desalted and transferred to a 384-element SpectroCHIP array. Allele detection was performed using MALDI-TOF MS. The mass spectrograms were analyzed by the MassARRAY Typer software (Sequenom). SNPs with MAF <1%, a call rate <95%, and deviation from Hardy-Weinberg equilibrium (P < 0.05) in the control subjects were excluded from further association analysis.
Massarray iplex1 system
Manufactured by Labcorp
Sourced in China
The MassArray iPLEX1 system is a high-throughput genotyping platform that utilizes matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry technology to analyze genetic variations. The system is designed to perform multiplexed single nucleotide polymorphism (SNP) genotyping and other genetic analyses.
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Lab products found in correlation
2 protocols using massarray iplex1 system
1
Genotyping of 8 SNPs using MassARRAY
2
Genotyping of 8 SNPs using MassARRAY
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The detection primers for the 8 SNPs were designed using the MassARRAY Assay Design 3.0 software (Sequenom). Approximately 15-20 ng of genomic DNA for each sample was used to genotype. Eight SNPs were genotyped by using the Sequenom MassArray iPLEX1 system (the Key Laboratory of Dermatology, Ministry of Education, China). The DNA samples were ampli ed by multiplex PCR reactions, and then the PCR products were used for locus-speci c singlebase extension reactions. The resulting products were desalted and transferred to a 384-element SpectroCHIP array. Allele detection was performed using MALDI-TOF MS. The mass spectrograms were analyzed by the MassARRAY Typer software (Sequenom). SNPs with MAF <1%, a call rate <95%, and deviation from Hardy-Weinberg equilibrium (P < 0.05) in the control subjects were excluded from further association analysis.
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