One gram of PGP is diluted using 9 ml physiological NaCl (Merck) solution of 0.85% until dilution to 10 − 3. 100 μl of each dilution was inserted into the Petri dish and then poured yeast and mold agar/YMA (Merck) and incubated for 48 h at room temperature (25°C–28°C) and then replicated three times. Yeast isolates were sequenced according to internal transcribed spacer (ITS) region with the primers of ITS1 and ITS4, aligned by MEGA X, and analyzed with Basic Local Alignment Search Tools (BLAST) algorithm.
Physiological nacl
Manufactured by Merck Group
Physiological NaCl is a sterile, isotonic saline solution that contains 0.9% sodium chloride. It is a commonly used laboratory reagent for various applications, such as cell culture, buffer preparation, and dilution of samples. The solution mimics the osmotic pressure and ion composition of human body fluids, making it suitable for maintaining the physiological environment of cells and tissues.
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Lab products found in correlation
Mg powder, by Merck Group (1 mentions)
Mayer s reagent, by Merck Group (1 mentions)
Concentrated hcl, by Merck Group (1 mentions)
Concentrated h2so4, by Merck Group (1 mentions)
Carbopol, by Merck Group (1 mentions)
2 n hydrochloric acid, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Propylene glycol, by Merck Group (1 mentions)
1 ml syringe, by Terumo (1 mentions)
Fecl3, by Merck Group (1 mentions)
Methylparaben, by Merck Group (1 mentions)
2 protocols using physiological nacl
1
Yeast Isolation and Identification Protocol
2
Marine Sponge-Derived Bioactive Compounds
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The tools used in this study were rotary evaporator (Heidolp), maceration bottle, analytical balance (Kenko), 1 mL syringe (Terumo), stopwatch, caliper (Tricle Brand), mouse cage, glass beaker (Pyrex), measuring cup (Pyrex), porcelain dish, pH meter (Milwaukee), brookfield viscometer, stove, stirring rod (Pyrex), measuring flask (Pyrex), dropper (Pyrex), glass object, filter paper, pycnometer (Pyrex), centrifuge (Hettich) , petri dish (Pyrex), mortar and pestle, analytical balance (Kenko).
The materials used were marine sponge (Axinella carteri) from Natuna Waters, methanol (Merck), carbopol (Merck), 1% carrageenan (Merck), diclofenac sodium as a positive control (Voltaren®), aquadest (Brataco), propylene glycol (Merck). , TEA (Merck), methyl paraben (Merck), 0.9% physiological NaCl (Merck), Mayer's reagent (Merck), concentrated HCl (Merck), chloroform (Merck), ammonia 10% (Merck), mg powder (Merck ), 2N hydrochloric acid (Merck), 1% FeCl3 (Merck), acetic acid (Merck), concentrated H2SO4 (Merck), chloroform (Merck), mice.
The materials used were marine sponge (Axinella carteri) from Natuna Waters, methanol (Merck), carbopol (Merck), 1% carrageenan (Merck), diclofenac sodium as a positive control (Voltaren®), aquadest (Brataco), propylene glycol (Merck). , TEA (Merck), methyl paraben (Merck), 0.9% physiological NaCl (Merck), Mayer's reagent (Merck), concentrated HCl (Merck), chloroform (Merck), ammonia 10% (Merck), mg powder (Merck ), 2N hydrochloric acid (Merck), 1% FeCl3 (Merck), acetic acid (Merck), concentrated H2SO4 (Merck), chloroform (Merck), mice.
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