HPLC-DAD for analysis was performed on Gilson GX-271 Liquid Handler with system 322 H2 Pump connected to a 171 Gilson Diode Array Detector and fraction collector. A XBridge BEH C18 XP Column (130 Å, 2.5 μm, 4.6 mm × 100 mm) with XBridge BEH C18 XP VanGuard Cartridge (130 Å, 2.5 μm, 3.9 mm × 5 mm) was used for analytical run with a flow rate of 0.8 ml/min. HPLC-grade water with 0.5% formic acid (solvent A) and HPLC-grade acetonitrile with 0.5% formic acid (solvent B) were used with the following gradients: 0 min, 20% Solvent B; 2 min, 20% Solvent B; 15 min, 95% Solvent B; 18 min, 95% Solvent B; 20 min, 20% Solvent B. Data acquisition and procession for the HPLC-DAD were controlled by TRILUTION LC V3.0.
Diode array detector
Manufactured by Gilson
The Diode Array Detector is a type of spectrophotometric detector used in high-performance liquid chromatography (HPLC) systems. It measures the absorbance of light by the analytes eluting from the HPLC column at multiple wavelengths simultaneously, providing detailed spectral information about the detected compounds.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using diode array detector
1
HPLC-DAD Analysis of Bioactive Compounds
2
HPLC-DAD Analysis of Compounds
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