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2 protocols using secondary goat anti rabbit as014

1

Alantolactone-Mediated Apoptosis Signaling

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Alantolactone (purity ≥ 98% [HPLC], CAS:546-43-0) was purchased from Chengdu Biopurify Phytochemicals Ltd (China). SP600125 (an inhibitor of c-Jun N-terminal kinase [JNK]), PD98059 (an inhibitor of extracellular-regulated protein kinase [ERK]), and SB203580 (an inhibitor of p38 MAPK) were purchased from MedChemExpress (Monmouth Junction, NJ, USA). Propidium iodide (PI) and annexin V/PI were purchased from BD Pharmingen (San Diego, CA, USA). Transwell plates and Matrigel were purchased from Corning Incorporated (Corning, NY, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). Primary antibodies against cleaved caspase-3 (#9661S), caspase-3 (#9662S), cyclin-B1 (#4138S), cyclin-E (#4129S), cyclin-dependent kinase 4 (CDK4) (#12790), JNK1/2 (#9255), p-JNK1/2 (#9252), p38 (#9212), p-p38 (#9215), ERK1/2 (#4348), p-ERK1/2 (#4377), and β-actin (#4970) were supplied by Cell Signaling Technology (CST, Danvers, MA, USA). p21 (#A16633), p53 (#A3185), c-Jun (#A11378), p-c-Jun (#AP0048), and secondary goat anti-rabbit (#AS014) or rabbit antimouse (#AS003) antibodies were supplied by Abclonal (Wuhan, China). Anti-Bcl-2 (#ab196495) and anti-BAX (#ab53154) antibodies were obtained from Abcam (Cambridge, MA, USA).
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2

Alantolactone-Mediated Apoptosis Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Alantolactone (purity ≥ 98% [HPLC], CAS:546-43-0) was purchased from Chengdu Biopurify Phytochemicals Ltd (China). SP600125 (an inhibitor of c-Jun N-terminal kinase [JNK]), PD98059 (an inhibitor of extracellular-regulated protein kinase [ERK]), and SB203580 (an inhibitor of p38 MAPK) were purchased from MedChemExpress (Monmouth Junction, NJ, USA). Propidium iodide (PI) and annexin V/PI were purchased from BD Pharmingen (San Diego, CA, USA). Transwell plates and Matrigel were purchased from Corning Incorporated (Corning, NY, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). Primary antibodies against cleaved caspase-3 (#9661S), caspase-3 (#9662S), cyclin-B1 (#4138S), cyclin-E (#4129S), cyclin-dependent kinase 4 (CDK4) (#12790), JNK1/2 (#9255), p-JNK1/2 (#9252), p38 (#9212), p-p38 (#9215), ERK1/2 (#4348), p-ERK1/2 (#4377), and β-actin (#4970) were supplied by Cell Signaling Technology (CST, Danvers, MA, USA). p21 (#A16633), p53 (#A3185), c-Jun (#A11378), p-c-Jun (#AP0048), and secondary goat anti-rabbit (#AS014) or rabbit antimouse (#AS003) antibodies were supplied by Abclonal (Wuhan, China). Anti-Bcl-2 (#ab196495) and anti-BAX (#ab53154) antibodies were obtained from Abcam (Cambridge, MA, USA).
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