3 morpholinopropanesulfonic acid mops

Glucose oxidase (GOx) from Aspergillus niger was purchased from Toyobo (Tokyo, Japan). We determined the concentration of GOx spectrophotometrically using the absorption coefficient of 18240 M -1 cm -1 at 460 nm. 11 ε-Poly-L-lysine was kindly donated by JNC (Tokyo). The sample consisted mainly of 25 -35 L-lysine residues, and its weight-average molecular weight was reported to be 4700 Da. 9, 12 Poly-L-lysine hydrobromide (αPL, MW = 15000 -30000), glycol chitosan (GCh), potassium ferrocyanide, hydroquinone (HQ), D-glucose, and 25% glutaraldehyde solution were purchased from Wako Pure Chemical Industries (Osaka, Japan). Bovine serum albumin (BSA) and poly(allylamine hydrochloride) (PAA, MW = 15000) were purchased from Sigma-Aldrich (St. Louis, MO). 2-Morpholinoethanesulfonic acid (MES) and 3-morpholinopropanesulfonic acid (MOPS) were purchased from Dojindo Laboratories (Mashiki, Japan). They were used without further purification. Other chemicals were of reagent grade and were used as received.

Glucose oxidase (GOx) from Aspergillus niger was purchased from Toyobo (Tokyo, Japan). The concentration of GOx was determined spectrophotometrically using the absorption coefficient of 18240 M -1 cm -1 at 460 nm. 24 PHMB and catalase were purchased from Nacalai Tesque (Kyoto, Japan). Ethylenediamine-N,N,N,′N′,-tetraacetic acid, disodium salt, dehydrate (EDTA), 2-morpholinoethanesulfonic acid (MES), 3-morpholinopropanesulfonic acid (MOPS) and N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS) were purchased from Dojindo Laboratories (Mashiki, Japan). Poloxamer 407 was purchased from Sigma-Aldrich (St. Louis, MO). They were used without further purification. Other chemicals were of reagent grade and were used as received.