Eyes fixed in 4% paraformaldehyde for 24 h at room temperature were then dehydrated and embedded in paraffin. Orientation of the serial sections (8 μm) was assured by controlling that the cuttings passed through the optic nerve head and the cutting marker. Sections were stained with hematoxilin and eosin, and the total thickness of the retina and of both the inner and outer nuclear layers were measured at × 20 magnification using the ScanScope Digital Scanner and the Image Scope software (Aperio Technologies, Vista, CA). Images were taken at equivalent retinal eccentricities from the optic nerve head, and three measurements were taken on each retina within 1 mm of the optic nerve.
Scanscope digital scanner
Manufactured by Leica
Sourced in United States
The ScanScope Digital Scanner is a high-resolution scanning device designed for digitizing slides and other materials. It captures detailed images of specimens with precision and accuracy.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using scanscope digital scanner
1
Retinal Layer Thickness Measurement
2
Collagen Quantification in Paraffin Sections
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Paraffin sections of methanol-Carnoy fixed tissue were stained for collagens I, III, and IV with specific antibodies (catalog 1310-01, 1330-01, and 1340-01, Southern Biotech, Birmingham, AL, USA, respectively). The secondary antibodies consisted of peroxidase-coupled rabbit anti-goat IgG (catalog P044901-2, Agilent/Dako, Santa Clara, CA, USA). Histological morphometry was performed using a ScanScope digital scanner (Aperio Technologies, Inc., Vista, CA, USA), and results were expressed as mean ± SEM% area.
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