FTIR spectra were collected using a Thermo Nicolet Nexus 470 FTIR spectrometer (GMI, MN, USA) with a deuterated triglycine sulfate (DTGS) detector four days after hydrogel preparation. Hydrogel samples were deposited on disposable KBr IR cards (Sigma-Aldrich, Rehovot, Israel) and dried under a vacuum. Measurements were taken using 4 cm−1 resolution and by averaging 2000 scans. The absorbance maxima values were determined using the OMNIC analysis program (Nicolet). The obtained transmittance spectra were smoothed by applying the Savitzky–Golay function to eliminate noise and operating the second derivative transformation on the spectra using the Peakfit software version 4.12 (SYSTAT Software Inc., Richmond, CA, USA).
Peakfit software version 4
Manufactured by Grafiti LLC
Sourced in United States
PeakFit software version 4.12 is a data analysis tool used for curve fitting and peak separation. It provides functionalities for analyzing spectroscopic data, chromatographic data, and other types of experimental data.
Automatically generated - may contain errors
Lab products found in correlation
Omnic software, by Thermo Fisher Scientific (3 mentions)
Nicolet is 5 spectrometers, by Thermo Fisher Scientific (2 mentions)
Kbr infrared sample cards, by Merck Group (1 mentions)
16 pc spectrometer, by PerkinElmer (1 mentions)
Ft ir 4600, by Jasco (1 mentions)
Atr pro one accessory, by Jasco (1 mentions)
Kbr ir sample cards, by Merck Group (1 mentions)
Dxr2xi raman spectrometer, by Thermo Fisher Scientific (1 mentions)
Nano inxider instrument, by Xenocs (1 mentions)
Attenuated total reflectance accessory, by Thermo Fisher Scientific (1 mentions)
14 protocols using peakfit software version 4
1
FTIR Spectroscopy Analysis of Hydrogels
2
FTIR Analysis of Peptide Structures
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FTIR spectroscopy was performed with 30 μl samples of peptide solutions, deposited onto disposable KBr infrared sample cards (Sigma-Aldrich, Rehovot, Israel), which were then allowed to dry under vacuum. Transmission infrared spectra were collected using a nitrogen-purged Nexus 470 FTIR spectrometer (Nicolet, Offenbach, Germany) equipped with a deuterated triglycine sulfate (DTGS) detector. Measurements were made by averaging 64 scans in 4 cm−1 resolution. The amide I region was deconvoluted by the second derivative method using the Peakfit software version 4.12 (Systat Software Inc., San Jose, CA, USA).
3
Infrared Spectroscopic Analysis of Chitosan-Polyphenol Interactions
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The infrared spectra of the films were determined with a Fouriertransform infrared spectrometer (FTIR; Perkin Elmer 16 PC spectrometer, Boston, USA), based on the method reported by Rubilar, Cruz, Silva et al. (2013) , using the attenuated total reflectance mode. Each spectrum results from 16 scans at 4 cm -1 resolution in the spectral range from 650 to 4000 cm -1 . All readings were performed at room temperature (20 °C). FTIR spectroscopy was used as a tool to investigate the interactions between chitosan and grape seed polyphenols and carvacrol by measuring the absorbance in the 650-4000 cm -1 wavenumber range at 4 cm -1 resolution. In the case of overlapping peaks, deconvolution was performed to calculate the contribution of the individual peaks using Peakfit software version 4.12 (SYSTAT Software Inc., Richmond, CA, USA). Deconvolution was used to estimate the peak area related to the specific vibration. The film spectra were deconvoluted with a smoothing filter of 15%. Each spectrum was baselinecorrected and the absorbance normalized between 0 and 1.
4
Thermal Analysis of Dried Films
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Thermogravimetric analysis of dried films was carried out using a Mettler Toledo thermogravimetric analyzer with differential scanning calorimetric capability, mod. TGA/DSC1 (Schwerzenbach, Switzerland). Samples (7–9 mg) were heated in an aluminum pan from 30 to 600 °C at a rate of 10 °C/min under a nitrogen atmosphere (30 mL/min). The first derivative of the TGA curve (DTG) was calculated using the PeakFit software version 4.12 (SYSTAT Software, Richmond, CA, USA). Determinations were done in triplicate.
5
Attenuated Total Reflectance-FTIR Analysis of Chickpea Flour Films
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The attenuated total reflectance-FTIR spectra of chickpea flour films were obtained using an ABB Bomen spectrometer mod. MB102 (Québec, QC, Canada) was in the range of 400 to 4000 cm−1 wavenumber. Each film spectrum was the result of 16 scans at 1 cm−1 resolution. Determinations were done in triplicate. Analysis of spectral data was performed with the PeakFit software version 4.12 (SYSTAT Software, Richmond, CA, USA). All spectra were base-line corrected and smoothed with a Savitsky-Golay function to remove possible noise before further data analysis. The identification of band positions was accomplished by Fourier deconvolution. Parameters of Fourier deconvolution were selected after several trials to give reasonable fits. Films spectra were deconvoluted with a Gaussian response function deconvolution filter constant of 74.9%.
6
Attenuated Total Reflectance FTIR Analysis
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The attenuated total reflectance-Fourier transform infrared spectra of films were obtained with a Jasco FT/IR-4600 equipped with the ATR Pro One accessory with an internal reflectance element of ZnSe (wave number range of 15,000–550 cm−1) (Jasco Corporation, Tokyo, Japan). Determinations were conducted in triplicate. The spectra were measured between 400 and 4000 cm−1 by co-adding 16 scans at 1 cm−1 resolution. Data treatments were performed with PeakFit software version 4.12 (SYSTAT Software, Richmond, CA, USA), according to Díaz et al. [3 (link)].
7
FTIR Analysis of Hydrogel Samples
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FTIR spectra were collected 4 days after hydrogel preparation using a Nicolet Nexus 470 FTIR spectrometer with a DTGS (deuterated triglycine sulphate) detector. Hydrogel samples were placed onto disposable KBr IR sample cards (Sigma–Aldrich) and vacuum dried. Measurements were performed using 4 cm−1 resolution and by averaging 2000 scans. The absorbance maxima values were determined using the OMNIC analysis program (Nicolet). The obtained transmittance spectra were smoothed by applying the Savitzky–Golay function to eliminate noise and operating the second derivative transformation on the spectra using Peakfit software version 4.12 (SYSTAT Software Inc., Richmond, CA).
8
Raman Spectroscopic Analysis of Surimi Gel
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The protein structure of the BD surimi gel was analyzed by Raman spectroscopy (DXR2xi Raman spectrometer; Thermo Fisher Scientific, USA), according to the method of Herrero et al. (18 (link)). The samples were scanned three times in the range of 50–3400 cm–1, using a λ = 785 nm semiconductor laser operated at room temperature. After smoothing and baseline correction, the spectral peaks were differentiated and imitated using the Peakfit software version 4.1.2 (Systat Software, USA). The intensities of the Raman signals were normalized relative to the phenylalanine peak at 1003 cm–1, as it is insensitive to the microenvironment.
9
Solid-state 13C NMR Characterization
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Solid-state nuclear magnetic resonance (13C NMR) was performed using a Bruker Avance III 400 MHz (1H/proton resonance) Wide Bore (Bruker-AXS, Karlsruhe, Germany) equipped with a 4-mm broadband double-resonance CP/MAS probe. Each film sample (500 mg) was placed into the rotor and introduced to the center of a magnetic field. The spectra were decomposed using PeakFit software version 4.0 (Systat Software Inc, San Jose, CA, USA).
10
Analytical Techniques for Protein Characterization
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The data were presented as the means ± standard deviations (SD) of three replicate determinations. Statistical analysis was performed using SPSS (20.0) software. Significant differences (p 0.05) between means were identified using Duncan’s multiple range test. The analysis of the Fourier transform infrared spectroscopy and fluorescence spectra was conducted using OMNIC software (Thermo Fisher Scientific, Waltham, MA, USA) and Peak Fit software version 4.0 (Systat Software Inc., San Jose, CA, USA).
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