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Prescreened fbs

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Prescreened FBS is a high-quality fetal bovine serum that has been thoroughly screened and tested to ensure consistent performance in cell culture applications. It provides essential nutrients and growth factors to support the growth and proliferation of a wide range of cell types.

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Lab products found in correlation

Ficoll paque, by Corning (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)

3 protocols using prescreened fbs

1

Ficoll Isolation of Mononuclear Cells

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All processing was completed using BSL 2 procedures. Prior to cell isolation, a blood aliquot was diluted with one part 10% RPMI to one part whole blood in a sterile 50 mL conical tube. Diluted blood was layered over Ficoll-Paque® (Lymphocyte Separation Medium, Cellgro) at a ratio of 3 mL blood to 1 mL separation medium. Blood was pipetted slowly down the side of the 50 mL conical to overlay the Ficoll-Paque. Samples were centrifuged at 20°C for 30 min at 900 g with no brake. After centrifugation the mononuclear cell layer was removed and transferred to a new 50 mL conical. The tube was filled with 5% RPMI and centrifuged for 5 min at 500 g at 20°C, with the brake. Cells were washed with 5% RPMI, and then resuspended in 10% RPMI. Cells were frozen in 1 × 107 cells/mL aliquots in freeze media, 10% DMSO (Sigma) and 90% sterile-filtered prescreened FBS (Gemini Bioproducts). Cells were kept in long-term storage in −150° freezers.
Carey A.J., Hope J.L., Mueller Y.M., Fike A.J., Kumova O.K., van Zessen D.B., Steegers E.A., van der Burg M, & Katsikis P.D. (2017). Public Clonotypes and Convergent Recombination Characterize the Naïve CD8+ T-Cell Receptor Repertoire of Extremely Preterm Neonates. Frontiers in Immunology, 8, 1859.
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2

Isolation of Mononuclear Cells from Cord Blood

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All processing was completed using BSL 2 procedures. Prior to cell isolation, a cord blood aliquot was diluted with one part 10% RPMI to one part whole blood in a sterile 50 mL conical tube. Diluted blood was layered over Ficoll-Paque® (Lymphocyte Separation Medium, Cellgro) at a ratio of 3-mL blood to 1-mL separation medium. Blood was pipetted slowly down the side of the 50 mL conical to overlay the Ficoll-Paque. Samples were centrifuged at 20°C for 30 min at 900g with no brake. After centrifugation the mononuclear cell layer was removed and transferred to a new 50mL conical. The tube was filled with 5% RPMI and centrifuged for 5 minutes at 500g at 20°C, with the brake. Cells were washed with 5% RPMI, and then resuspended in 10% RPMI. Cells were frozen in 10×106 cells/mL aliquots in freeze media, 10% DMSO (Sigma) and 90% sterile-filtered prescreened FBS (Gemini Bioproducts). Cells were kept in long term storage in −150 degree freezers.
Fike A.J., Nguyen L.T., Kumova O.K, & Carey A.J. (2017). Characterization of CD31 expression on murine and human neonatal T lymphocytes during development and activation. Pediatric research, 82(1), 133-140.
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3

Isolation of Mononuclear Cells from Cord Blood

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All processing was completed using BSL 2 procedures. Prior to cell isolation, a cord blood aliquot was diluted with one part 10% RPMI to one part whole blood in a sterile 50 mL conical tube. Diluted blood was layered over Ficoll-Paque® (Lymphocyte Separation Medium, Cellgro) at a ratio of 3-mL blood to 1-mL separation medium. Blood was pipetted slowly down the side of the 50 mL conical to overlay the Ficoll-Paque. Samples were centrifuged at 20°C for 30 min at 900g with no brake. After centrifugation the mononuclear cell layer was removed and transferred to a new 50mL conical. The tube was filled with 5% RPMI and centrifuged for 5 minutes at 500g at 20°C, with the brake. Cells were washed with 5% RPMI, and then resuspended in 10% RPMI. Cells were frozen in 10×106 cells/mL aliquots in freeze media, 10% DMSO (Sigma) and 90% sterile-filtered prescreened FBS (Gemini Bioproducts). Cells were kept in long term storage in −150 degree freezers.
Fike A.J., Nguyen L.T., Kumova O.K, & Carey A.J. (2017). Characterization of CD31 expression on murine and human neonatal T lymphocytes during development and activation. Pediatric research, 82(1), 133-140.
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