Luminex mouse 32 plex mcytmag 70k px32

Manufactured by Merck Group

The Luminex Mouse 32-plex (MCYTMAG-70K-PX32) is a multiplex assay kit that allows for the simultaneous quantification of 32 different mouse cytokines and chemokines from a single sample. The kit utilizes Luminex's xMAP technology to measure analytes in a fluid suspension, enabling high-throughput and multiplexed analysis.

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Lab products found in correlation

Zombie nir, by BioLegend (2 mentions) Cd11c pe cy7, by BioLegend (2 mentions) Ly6c fitc, by BioLegend (2 mentions) Ic fixation buffer, by Thermo Fisher Scientific (2 mentions) Cd11b, by Merck Group (2 mentions) Ly 6g, by Merck Group (2 mentions) Ly6g bv650, by BioLegend (1 mentions)

Close spelling variants of this product

MCYTMAG-70K-PX32 (19 citations)

2 protocols using luminex mouse 32 plex mcytmag 70k px32

Comprehensive Immune Characterization of BALF

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BAL fluid was collected from each animal through cannulation of the exposed trachea, and flushing twice with .7mL of PBS. BALF samples were centrifuged at 500xG for 5 minutes, and supernatant was immediately frozen for later cytokine analyses. For flow cytometry, pelleted cells were resuspended in FACS (PBS + 5% FBS) buffer for staining and with Zombie NIR (Biolegend, 423105, SanDiego, CA), CD45, Alexa Fluor 532 (eBioscience, 58-0459-42,San Diego, CA), CD11c, PE-Cy7 (Biolegend, 117317, SanDiego, CA), CD11B, BV480 (BD Biosciences, 566117, SanJose, CA), SIGLEC F, AF700 (eBioscience, 56-1702-80, SanDiego, CA), Ly-6c, FITC (Biolegend, 128005, San Diego, CA),and Ly-6G, BV650 (Biolegend, 127641, San Diego, CA) and then fixed in IC-fixation buffer (eBioscience, 00-8222-49, San Diego, CA). Samples were run on a Cytek Aurora Borealis at the University of Virginia flowcytometry core. Neutrophils are Zombie NIR−, CD45+,CD11C−, CD11B+, and Ly-6G+; eosinophils are Zombie NIR−,CD45+, CD11C−, CD11B+, and Siglec-F+; inflammatory monocytes are Zombie NIR−, CD45+, CD11C−, CD11B+, Ly-6G−, and Ly-6C+.
Cytokine analyses were performed via Luminex Mouse 32-plex (MCYTMAG-70K-PX32, Millipore-sigma). Samples were run following manufacturers protocol after an 18-hour incubation before being run on Luminex® analyzer (MAGPIX®).

Donlan A.N., Sutherland T.E., Marie C., Preissner S., Bradley B.T., Carpenter R.M., Sturek J.M., Ma J.Z., Moreau G.B., Donowitz J.R., Buck G.A., Serrano M.G., Burgess S.L., Abhyankar M.M., Mura C., Bourne P.E., Preissner R., Young M.K., Lyons G.R., Loomba J.J., Ratcliffe S.J., Poulter M.D., Mathers A.J., Day A., Mann B.J., Allen J.E, & Petri WA J.r. (2021). IL-13 is a driver of COVID-19 severity. medRxiv.

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Analyzing Immune Cell Subsets in BALF

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BAL fluid was collected from each animal through cannulation of the exposed trachea and flushed twice with 0.7mL of PBS. BALF samples were centrifuged at x500 g for 5 minutes, and supernatant was immediately frozen for later cytokine analyses. For flow cytometry, pelleted cells were resuspended in FACS (PBS + 5% FBS) buffer for staining and with Zombie NIR (BioLegend, 423105), CD45, Alexa Fluor 532 (eBioscience, 58-0459-42), CD11c, PE-Cy7 (BioLegend, 117317), CD11B, BV480 (BD Biosciences, 566117), SIGLEC F, AF700 (eBioscience, 56-1702-80), Ly-6c, FITC (BioLegend, 128005), and Ly-6G, BV650 (BioLegend, 127641) and then fixed in IC-fixation buffer (eBioscience, 00-8222-49). Samples were run on a Cytek Aurora Borealis at the University of Virginia flow cytometry core. Neutrophils are Zombie NIR−, CD45+, CD11C−, CD11B+, and Ly-6G+; eosinophils are Zombie NIR−, CD45+, CD11C−, CD11B+, and Siglec-F+; and inflammatory monocytes are Zombie NIR−, CD45+, CD11C−, CD11B+, Ly-6G−, and Ly-6C+.
Cytokine analyses were performed via Luminex Mouse 32-plex (MCYTMAG-70K-PX32, MilliporeSigma). Samples were run following manufacturers protocol after an 18-hour incubation before being run on Luminex analyzer (MAGPIX).

Donlan A.N., Sutherland T.E., Marie C., Preissner S., Bradley B.T., Carpenter R.M., Sturek J.M., Ma J.Z., Moreau G.B., Donowitz J.R., Buck G.A., Serrano M.G., Burgess S.L., Abhyankar M.M., Mura C., Bourne P.E., Preissner R., Young M.K., Lyons G.R., Loomba J.J., Ratcliffe S.J., Poulter M.D., Mathers A.J., Day A.J., Mann B.J., Allen J.E, & Petri WA J.r. (2021). IL-13 is a driver of COVID-19 severity. JCI Insight, 6(15), e150107.

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