Ges 1

Manufactured by Shanghai Institute of Biochemistry and Cell Biology

Sourced in China

GES-1 is a laboratory equipment designed for gene expression studies. It is a high-quality instrument that enables researchers to quantify and analyze gene expression levels in biological samples. The core function of GES-1 is to provide accurate and reliable data for gene expression analysis.

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Lab products found in correlation

13 protocols using ges 1

H. pylori Infection of Gastric Cells

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HEK293T cells and human GAC cell line AGS was obtained from ATCC. Immortalized normal human gastric epithelial cell line GES-1 and human GAC cell lines BGC-823 and SGC-7901 were obtained from Shanghai Institute of Cell Biology, China Academy of Sciences (Shanghai, China). HEK293T cells were cultured in DMEM medium, and other cells were cultured in RPMI-1640 medium supplemented with 10% FBS at 37°C in 5% CO₂ in a 25-cm² flask.
The wild-type H. pylori strains 26695 were obtained from ATCC. The bacteria were grown on CDC anaerobe blood agar plates (BD) and were incubated at 37°C for 2 days in an anaerobic jar containing a gas mixture of 5% O₂, 10% CO₂, and 85% N₂ (DU Scientific).
When the cells reached 80% confluency, the medium was replaced with antibiotic-free medium and H. pylori was added to the flask at a multiplicity of infection of 100:1. At 18 h after H. pylori induction, the cells were lysed to extract total RNA.

Liu W., Song N., Yao H., Zhao L., Liu H, & Li G. (2015). miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 21, 2718-2725.

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Culturing Human Gastric Cell Lines

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Human gastric cancer cell line SGC-7901, cisplatin resistant cell line SGC-7901/DDP and normal gastric epithelial cell line GES-1 were purchased from the Shanghai Institute of Cell Biology (Shanghai, China). Cells were cultured in PRIM-1640 (GIBCO, Rockville, USA) supplemented with 10% fetal bovine serum (GIBCO, Rockville, USA) and 1% antibiotics and grown at 37 °C in humidified air with 5% CO 2 .

Yan J., Zhang Y., She Q., Li X., Peng L., Wang X., Liu S., Shen X., Zhang W., Dong Y., Lu J, & Zhang G. (2017). Long Noncoding RNA H19/miR-675 Axis Promotes Gastric Cancer via FADD/Caspase 8/Caspase 3 Signaling Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 42(6).

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Culturing Immortalized Gastric Cell Lines

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The immortalized normal gastric mucosal epithelial cell line (GES-1) and human GC cell lines MKN-45, BGC-823, SGC-7901, MKN-28, and AGS were purchased from Shanghai Institute of Cell Biology (Shanghai, China). Cells were cultured in RPMI 1640 (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and supplemented with 10% fetal bovine serum (FBS) at 37 °C in a humidified atmosphere with 5% CO₂.

Li W., Li J., Mu H., Guo M, & Deng H. (2019). MiR-503 suppresses cell proliferation and invasion of gastric cancer by targeting HMGA2 and inactivating WNT signaling pathway. Cancer Cell International, 19, 164.

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Gastric Cancer Cell Line Irradiation

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Three human gastric adenocarcinoma cell lines (BGC823, SGC7901, and MKN45) and an immortalized human gastric mucosa cell line (GES-1) were purchased from the Shanghai Institute of Cell Biology (Shanghai, People’s Republic of China) and maintained in Roswell Park Memorial Institute 1640 medium with 10% calf bovine serum, 50 units/mL penicillin, and 50 units/mL streptomycin in 5% CO₂ at 37°C. Radiotherapy was administered in vitro using a 4 MeV electron beam linear accelerator (Elekta, Stockholm, Sweden).

Cui F.B., Liu Q., Li R.T., Shen J., Wu P.Y., Yu L.X., Hu W.J., Wu F.L., Jiang C.P., Yue G.F., Qian X.P., Jiang X.Q, & Liu B.R. (2014). Enhancement of radiotherapy efficacy by miR-200c-loaded gelatinase-stimuli PEG-Pep-PCL nanoparticles in gastric cancer cells. International Journal of Nanomedicine, 9, 2345-2358.

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Co-culture of Gastric Cell Lines with H. pylori

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Human normal gastric epithelial cell line (GES-1) was bought from Shanghai Institute of Cell Biology (Shanghai, China). Human GC cell lines (SGC-7901 and MKN45) and wild-type H. pylori strain 26695 were bought from American Type Culture Collection (ATCC, Manassas, VA, USA). Infection procedure was performed as described.18 (link) Cell lines SGC-7901 and MKN45 were propagated in DMEM (Gibco, Invitrogen, Waltham, MA, USA) with 10% FBS in a humidified incubator (5% CO₂ at 37℃). After cells grew to be approximately 80% confluent, they were co-cultured with HP at multiplicity of infection (M.O.I) of 100:1. After 12 h of infection, total RNA was extracted. All cell lines were maintained in RPMI 1640 that was supplemented in a humidified atmosphere of 95% air and 5% CO₂ with 10% fetal calf serum at 37℃, which were identified by authentication.

Xu X.C., Zhang W.B., Li C.X., Gao H., Pei Q., Cao B.W, & He T.H. (2018). Up-Regulation of MiR-1915 Inhibits Proliferation, Invasion, and Migration of Helicobacter pylori-Infected Gastric Cancer Cells via Targeting RAGE. Yonsei Medical Journal, 60(1), 38-47.

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Cell Culture of Gastric Cancer Lines

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Human gastric adenocarcinoma AGS cells (ATCC, Manassas, VA, USA) were grown in F-12k (ATCC). Other GC cells MKN-45, BGC-823, SGC-7901, MKN-28 and normal human gastric epithelial cell line GES-1 (Shanghai Institute of Cell Biology, China) were grown in RPMI-1640 (Gibco). All of these cells were supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin at 37°C with humidified 5% CO₂.

Liu F., Cao Q.H., Lu D.J., Luo B., Lu X.F., Luo R.C, & Wang X.G. (2015). TMEM16A overexpression contributes to tumor invasion and poor prognosis of human gastric cancer through TGF-β signaling. Oncotarget, 6(13), 11585-11599.

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Culturing Human Gastric Cell Lines

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The human GC cell lines (BGC823, MGC803, SGC7901, MKN45, AGS, and N87) and gastric immortalized epithelial cell line (GES1) were purchased from the Shanghai Institute of Cell Biology. All cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS) containing 100 units/mL penicillin and 100 mg/mL streptomycin (P/S, Gibco) at 37°C in a 5% CO₂ incubator.

Dong Z., Guo S., Wang Y., Zhang J., Luo H., Zheng G., Yang D., Zhang T., Yan L., Song L., Liu K., Sun Z., Meng X., Zheng Z., Zhang J, & Zhao Y. (2020). USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer. OncoTargets and therapy, 13, 8495-8510.

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Gastric Adenocarcinoma Cell Lines and Inhibitor Treatment

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Human gastric adenocarcinoma cell line MKN-45, BGC-823, MGC-803, SGC-7901, AGS and human gastric epithelial cell line GES-1 (Shanghai Institute of Cell Biology, China) were grown in F-12 k (ATCC) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin at 37 °C with humidified 5% CO₂. For inhibitor treatment, the cultured cells were incubated with 10 μmol/l BAY-117082 (Selleck, USA) for 48 h. Cells were collected in logarithmic growth phase for all experiments.

Cao Q.H., Liu F., Li C.Z., Liu N., Shu M., Lin Y., Ding L, & Xue L. (2018). Testes-specific protease 50 (TSP50) promotes invasion and metastasis by inducing EMT in gastric cancer. BMC Cancer, 18, 94.

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Cultivation of Human Gastric Cell Lines

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Five human GC cell lines (AGS, HGC-27, BGC-823, SGC-7901 and MGC80-3) and one immortalized gastric cell line (GES-1) were purchased from Shanghai Institute of Cell Biology (Shanghai, China). All cell lines were incubated in Dulbecco’s modified Eagle’s medium (Gibco-BRL, Carlsbad, CA, USA) with 10% fetal bovine serum (SAFC Biosciences Inc., Lenexa, KS, USA), 100 U/ml penicillin and 100 mg/ml streptomycin (Sigma-Aldrich, St. Louis, MO, USA).

XIE X., LIU X., ZHANG Q, & YU J. (2014). Overexpression of collagen VI α3 in gastric cancer. Oncology Letters, 7(5), 1537-1543.

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Maintaining Human Gastric Cell Lines

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Human gastric mucosal epithelial cell line GES‐1, gastric cancer cell lines SNU‐216, AGS, SGC‐7901 and BGC‐823 cells were obtained from Shanghai Institute of Cell Biology (Shanghai, China) and maintained in PRIM‐1640 (GIBCO, Rockville, MD, USA) with 10% fetal bovine serum (GIBCO), 100 U/mL penicillin, and 100 μg/mL streptomycin in humidified incubator with 5% CO₂ at 37°C.

Xiao J., Lai H., Wei S., Ye Z., Gong F, & Chen L. (2019). lncRNA HOTAIR promotes gastric cancer proliferation and metastasis via targeting miR‐126 to active CXCR4 and RhoA signaling pathway. Cancer Medicine, 8(15), 6768-6779.

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