Cal27, SCC-25, and SCC-15 cell lines, sourced from the American Type Culture Collection (ATCC, Manassas, VA), were utilized in our study. Cal27 cells were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, ATCC), while SCC-15 and SCC-25 cells were maintained in MEM NEAA medium. All cell cultures were incubated at 37 °C in a 5% CO2 atmosphere. Antibiotics used included a penicillin-streptomycin-gentamicin mix (100×, P1410-100 ml) and a penicillin-streptomycin solution (100×, P1400-100 ml) to prevent contamination.
Scc15
Manufactured by American Type Culture Collection
Sourced in United States, China, United Kingdom
The SCC15 is a laboratory equipment designed for the storage and preservation of biological samples. It is a cryogenic storage system that utilizes liquid nitrogen to maintain extremely low temperatures, ensuring the long-term viability of the stored materials.
Automatically generated - may contain errors
Lab products found in correlation
Scc25, by American Type Culture Collection (72 mentions)
Cal27, by American Type Culture Collection (72 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (53 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (30 mentions)
Scc15, by Thermo Fisher Scientific (22 mentions)
Dmem f12, by Thermo Fisher Scientific (19 mentions)
Cal27, by Thermo Fisher Scientific (13 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (13 mentions)
Scc 4, by American Type Culture Collection (11 mentions)
Scc25, by Thermo Fisher Scientific (11 mentions)
Hsc 3, by American Type Culture Collection (9 mentions)
Streptomycin, by Thermo Fisher Scientific (9 mentions)
Penicillin, by Thermo Fisher Scientific (7 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (5 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (5 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (5 mentions)
Hsc 3, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Merck Group (4 mentions)
Tca8113, by American Type Culture Collection (4 mentions)
Gibco s keratinocyte sfm, by Thermo Fisher Scientific (4 mentions)
156 protocols using scc15
1
Oral Squamous Cell Carcinoma Cell Lines
2
OSCC Cell Line Characterization
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Among three OSCC cell lines (SCC9, SCC15 and SCC25), SCC9 and SCC15 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) while SCC25 cells were from Procell Life Science & Technology Co., Ltd. (Wuhan, China). A healthy human oral keratinocyte (HOK) cell line was purchased from Binsui Biotechnology Co., Ltd. (Shanghai, China). All the cell lines were cultivated in Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Gaithersburg, MD, USA) and supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 100 U/ml penicillin/streptomycin under 5% CO2 at 37 °C. For circRNA characterization, 3 U/mg of RNase R from Epicentre Technologies (Madison, WI) and 2 mg/ml of Actinomycin D (Act D) from Sigma-Aldrich (St. Louis, MO) were used.
3
Acquisition and Maintenance of HNSCC Cell Lines
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Human HNSCC cell lines SCC9, SCC15, SCC25, CAL27 and HEK293T cells were obtained from ATCC (Rockville, MD, USA). UM1 and UM-SCC1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA). HSC3, HSC6 and CAL33 were kindly provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA). The characteristics of HNSCC cell lines are described in Table S1 . The UM-SCC1, HSC3, HSC6, CAL27, CAL33 and HEK293T cells were cultivated in Dulbecco's modified Eagle's medium (DMEM, Gibco, Rockville, MD, USA) supplemented with 10% fetal bovine serum (FBS, Gibco). The SCC9, SCC15, SCC25 and UM1 cells were maintained in DMEM-F12 (Gibco) supplemented with 10% FBS. All cells were incubated at 37°C in a humidified atmosphere containing 5% CO2. All cell lines were routinely tested for Mycoplasma by PlasmoTestTM Mycoplasma contamination detection kit (InvivoGen).
4
Cell Culture Conditions for HET-1A and SCC-15 Lines
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HET-1A (CRL-2692) and SCC-15 (CRL-1623) cell lines were purchased from ATCC (American Type Culture Collection, Wesel, Germany) and were cultured in Dulbecco's modified Eagle's Medium (DMEM) supplemented with 400 ng/ml hydrocortisone (H4001, Thermo Fisher Scientific, MA, USA), 10% fetal bovine serum (FBS, 10270106, Thermo Fisher Scientific, MA, USA), 100 U/ml penicillin and 100 µg/ml streptomycin at 37°C with 5% CO2.
5
Culturing Oral Cancer Cell Lines
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Human normal oral epithelial cells (HNOECs), SCC-15, sCC-25, and CAL-27 cells were cultured in complete medium (DMED [Gibco]) containing 10% fetal bovine serum (FBS, Invitrogen, California, USA), penicillin, and streptomycin (Solarbio, Beijing, China) at 37°C in 5% CO2, 95% air. HNOECs were purchased from Guide Chem (https://china.guidechem.com , Zhejiang, China) and SCC-15, sCC-25, and CAL-27 cells were obtained from ATCC (www.atcc.org , USA).
6
Culturing Human Oral Cancer Cell Lines
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Human OSCC cell lines SCC9, SCC15, and CAL27 were obtained from ATCC (Rockville, MD, USA). UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA). HSC3, HSC6 and CAL33 and normal oral keratinocytes (NOK) were kindly provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA). The HSC3, HSC6, CAL27, and CAL33 cells were cultivated in Dulbecco's modified Eagle's medium (DMEM, Gibco, Rockville, MD, USA) supplemented with 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA, USA). The SCC9, SCC15, and UM1 cells were maintained in DMEM-F12 (Gibco) supplemented with 10% FBS. NOK was grown in keratinocyte serum-free medium containing human recombinant epidermal growth factor and bovine pituitary extract (Life Technologies). All cells were incubated at 37°C in a humidified atmosphere containing 5% CO2.
7
OSCC Cell Characterization and Genetic Manipulation
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Human normal oral epithelial keratinocytes (HOK) cells were purchased from ScienCell. Human umbilical cord veins cells (HUVECs), SCC9, SCC15, SCC25 and UM1 were purchased from ATCC. The OSCC cell lines were cultured in Dulbecco’s modified Eagle’s medium/F12 (Gibco, New York, USA) with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin–streptomycin, incubated in 5% CO2 at 37℃. Anlotinib dihydrochloride was kindly provided by the Chia Tai Tianqing Pharmaceutical Group Co. Ltd. (Nanjing, China).
Lentivirus vectors containing METTL3 short hairpin RNA (shRNA) were purchased from OBiOc (Shanghai, China). Small interfering RNAs (siRNAs) (RiboBio, Guangzhou, China) targeting FGFR3 were designed to knockdown FGFR3 in OSCC. The indicated two shRNA and three siFGFR3 sequences are listed in Additional file5 : Table S1.
Lentivirus vectors containing METTL3 short hairpin RNA (shRNA) were purchased from OBiOc (Shanghai, China). Small interfering RNAs (siRNAs) (RiboBio, Guangzhou, China) targeting FGFR3 were designed to knockdown FGFR3 in OSCC. The indicated two shRNA and three siFGFR3 sequences are listed in Additional file
8
Culturing Human Head and Neck Cancer Cells
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Human HNSCC lines CAL27 and SCC15 were purchased from ATCC (Manassas, VA). The cells were maintained in complete Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA), 100 units of penicillin and 100 μg of streptomycin at 37°C in 5% CO2 as described [43 (link)–45 (link)], Unless indicated otherwise, all chemicals were purchased from Sigma-Aldrich (St. Louis, MO) or Thermo Fisher Scientific (Waltham, MA).
9
Culture of Human Oral Cancer Cells
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The human OSCC cell lines SCC9 and SCC15 were purchased from ATCC. Cells were cultured in DMEM (Gibco, Cat#11995500TB, Chengdu, China) with 10% fetal bovine serum (ExCell Bio, Inc., Chengdu, China) and incubated at 37 °C with 5% CO2. The medium of all cells was exchanged about every 1–2 days according to the cell density.
10
Culturing Human Oral Squamous Carcinoma Cell Lines
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A total of 6 human OSCC cell lines (UMSCC1 (UM1), SCC15, HSC4, CAL33, HSC3, and HSC6) were maintained at 37°C and 5% CO2 in DMEM/F12 (1:1) (Gibco, Invitrogen Corp) supplemented with 10% FBS and different concentrations of glutamine. The SCC15 and human normal oral keratinocyte (HOK) cell lines were purchased from ATCC. The HSC3 and HSC4 cell lines were gifts provided by YYYY Professor (XXXX University, China). The UM1 cell line was obtained from YYYY Professor (Oral and Maxillofacial Surgery department, XXXX University, China) and HSC‐6 and CAL33 cells were kindly donated by YYYY Professor (XXXX Institute of Dental and Craniofacial Research, USA).
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