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Hcc1395

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The HCC1395 is a cell line derived from a human breast cancer tumor. It is intended for use in cell culture and biological research applications.

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Lab products found in correlation

Mda mb 231, by American Type Culture Collection (17 mentions) Hcc1937, by American Type Culture Collection (16 mentions) Mcf 7, by American Type Culture Collection (15 mentions) Bt549, by American Type Culture Collection (15 mentions) Hcc38, by American Type Culture Collection (11 mentions) Hcc1806, by American Type Culture Collection (11 mentions) Skbr3, by American Type Culture Collection (9 mentions) Mda mb 436, by American Type Culture Collection (8 mentions) Mda mb 468, by American Type Culture Collection (7 mentions) Mcf 10a, by American Type Culture Collection (7 mentions) Hcc1143, by American Type Culture Collection (7 mentions) Hs578t, by American Type Culture Collection (7 mentions) Zr 75 1, by American Type Culture Collection (7 mentions) Mda mb 453, by American Type Culture Collection (6 mentions) Bt474, by American Type Culture Collection (6 mentions) Hcc1954, by American Type Culture Collection (6 mentions) Mda mb 157, by American Type Culture Collection (6 mentions) Bt 20, by American Type Culture Collection (5 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Hcc1187, by American Type Culture Collection (4 mentions)

34 protocols using hcc1395

1

Breast Cancer Cell Culture Protocols

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The study used the TNBC cell line MDA-MB-231 (ATCC HTB 26) and the HCC1395 (ATCC CRL-2324TM. The MDA-MB-231 cells were grown according to ATCC recommendations in DMEM/F-12 supplemented with 10% FBS, insulin 10 μg/mL, non-essential amino acids, sodium pyruvate, penicillin 5,000 I.U/mL, and streptomycin 5,000 μg/mL. The non-malignant cell line used in this study was MCF-10A (ATCC CRL-10317TM) which was cultured in DMEM/F12K containing 5% horse serum, insulin (10 μg/mL), hydrocortisone (0.5 mg/mL), EGF (20 ng/mL), penicillin (100 U/mL), and streptomycin (0.1 mg/mL). HCC1395 (ATCC CRL-2324TM) and HCC 1937 (ATCC CRL-2336™), two other TNBC cell lines, were grown in RPMI with 10% FBS, penicillin (100 U/mL), and streptomycin (0.1 mg/mL). The cell cultures were maintained at 37°C and 5% CO2.
Gupta S.R., Ta T.M., Khan M., Singh A., Singh I.K, & Peethambaran B. (2023). Identification and validation of a small molecule targeting ROR1 for the treatment of triple negative breast cancer. Frontiers in Cell and Developmental Biology, 11, 1243763.
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2

Breast Cancer Cell Line DNA Extraction

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Original cell line stocks for HCC38, HCC1143, HCC1187, HCC1954, HCC1937 and HCC1395 were obtained directly from the American Type Culture Collection (ATCC), cultured according to Neve et al [17 (link)], and passaged minimally prior to harvesting for the present study. For each respective cell line, normal matched DNA derived from B lymphoblastoid cell lines (HCC38 BL, HCC1143 BL, HCC1187 BL, HCC1954 BL, HCC1937 BL, HCC1395 BL) were purchased from ATCC. Breast cancer cells were harvested at approximately 75% confluency and genomic DNA was isolated using DNeasy Blood and Tissue Kit (Qiagen) according to the standard manufacturer protocol. The concentration of DNA was measured with the ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies).
Olsson E., Winter C., George A., Chen Y., Törngren T., Bendahl P.O., Borg Å., Gruvberger-Saal S.K, & Saal L.H. (2015). Mutation Screening of 1,237 Cancer Genes across Six Model Cell Lines of Basal-Like Breast Cancer. PLoS ONE, 10(12), e0144528.
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3

High-quality DNA Extraction from Cell Lines

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Cell lines were purchased from the American Type Culture Collection (ATCC). All cell lines were cultured according to ATCC handling guidelines at 37°C and 5% CO2. Cell counts were enumerated by Automated Cell Counter (BioRad, TC20), washed twice with Phosphate Buffered Saline (Gibco, 10010023), placed into 6 × 106 cell aliquots, flash frozen in liquid nitrogen, and stored at −80°C. Cancer cell lines: HCC1954 (ATCC, CRL-2338), H2009 (ATCC, CRL-5911), H1437 (ATCC, CRL-5872), and HCC1395 (ATCC, CRL-2324). Normal cell lines: HCC1954 BL (ATCC, CRL-2339), BL2009 (ATCC, CRL-5961), HCC1937, BL1437 (ATCC, CRL-5958), and HCC1395 BL (ATCC, CRL-2325). Utilizing the Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs, T3060), high molecular weight (HMW) DNA was extracted from 6 × 106 cells for all cell lines.
Keskus A., Bryant A., Ahmad T., Yoo B., Aganezov S., Goretsky A., Donmez A., Lansdon L.A., Rodriguez I., Park J., Liu Y., Cui X., Gardner J., McNulty B., Sacco S., Shetty J., Zhao Y., Tran B., Narzisi G., Helland A., Cook D.E., Chang P.C., Kolesnikov A., Carroll A., Molloy E.K., Pushel I., Guest E., Pastinen T., Shafin K., Miga K.H., Malikic S., Day C.P., Robine N., Sahinalp C., Dean M., Farooqi M.S., Paten B, & Kolmogorov M. (2024). Severus: accurate detection and characterization of somatic structural variation in tumor genomes using long reads. medRxiv.
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4

Cell Culture Maintenance Protocols

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Cells were cultured at 37℃ with 5% CO2 in a humidified incubator. MDA-MB-231, MDA-MB-468, MCF7, 4T1, and HCC1395 were from ATCC. MDA-MB-231 was authenticated by STR profiling. All of the cell lines were passaged less than 2 months after each thaw and tested to be free of mycoplasma contamination.
Liu H., Bai L., Huang L., Ning N., Li L., Li Y., Dong X., Du Q., Xia M., Chen Y., Zhao L., Li Y., Meng Q., Wang J., Duan Y., Ming J., Yuan A.Q, & Yang X.P. (2021). Bispecific antibody targeting TROP2xCD3 suppresses tumor growth of triple negative breast cancer. Journal for Immunotherapy of Cancer, 9(10), e003468.
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5

Nintedanib Modulates Signaling Pathways in Triple-Negative Breast Cancer

Cited 1 time
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MDA-MB-231, MDA-MB-468 and HCC-1395 cell lines were obtained from the ATCC (American Type Culture Collection, Rockville, MD, USA) and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, HyClone, Thermo Scientific, Waltham, MA, USA). Cell lysates treated with nintedanib were prepared and analyzed by western blot as previously reported.18 (link) Antibodies targeting p-JAK2 (Tyr1007/1008), JAK2, p-SRC (Tyr416), SRC, p-STAT3 (Tyr705), STAT3, survivin, poly (ADP-ribose) polymerase (PARP) and cleaved caspase 3 were purchased from Cell Signaling (Danvers, MA, USA). SHP-1, cyclin D1 and Mcl-1 antibodies were purchased from Abcam (Cambridge, MA, USA).
Liu C.Y., Huang T.T., Chu P.Y., Huang C.T., Lee C.H., Wang W.L., Lau K.Y., Tsai W.C., Chao T.I., Su J.C., Chen M.H., Shiau C.W., Tseng L.M, & Chen K.F. (2017). The tyrosine kinase inhibitor nintedanib activates SHP-1 and induces apoptosis in triple-negative breast cancer cells. Experimental & Molecular Medicine, 49(8), e366-.
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6

Overexpression and Silencing of FLRT2 in Breast Cell Lines

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Normal breast cell line MCF-10A and breast cancer cell lines MCF7, T47D, MDA-MB-231, HCC38, and HCC1395 were purchased from ATCC (Manassas, VA, USA) and cultured under the optimal conditions required according to instructions by ATCC. To over-express FLRT2 in cultured cells, a recombinant expression vector containing the open reading frame was designed and constructed as an OmicsLink ORF Expression Clone (Genecopoeia, Rockville, MD, USA). Pre-designed siRNAs for FLRT2 silencing were purchased from Bioneer (Daejeon, South Korea). The most effective siRNA concentration was determined by transfecting cells with 10, 40, and 80 nM siRNA. All transfection processes were performed with Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) by following the supplier’s protocol.
Bae H., Kim B., Lee H., Lee S., Kang H.S, & Kim S.J. (2017). Epigenetically regulated Fibronectin leucine rich transmembrane protein 2 (FLRT2) shows tumor suppressor activity in breast cancer cells. Scientific Reports, 7, 272.
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7

Culture of Triple Negative Breast Cell Lines

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Human triple negative breast cell lines HCC1806, HCC1143, and HCC1395 (ATCC) were maintained in RPMI-1640 medium and supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin at 37°C incubator with 5% CO2 and 95% humidity.
Kwan H.Y., Xu Q., Gong R., Bian Z, & Chu C.C. (2021). Targeted Chinese Medicine Delivery by A New Family of Biodegradable Pseudo-Protein Nanoparticles for Treating Triple-Negative Breast Cancer: In Vitro and In Vivo Study. Frontiers in Oncology, 10, 600298.
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8

Culturing TNBC and Monocyte Cell Lines

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Human TNBC cell lines MDA-MB-231 (ATCC, Manassas, VI, USA) and HCC1395 (ATCC), murine TNBC cell line 4T1 (ATCC) and human THP-1 monocytes (ATCC) were maintained in RPMI-1640 medium (Life Technologies, Grand Island, NY, USA) with 10% fetal bovine serum (FBS) (Life Technologies), 2 mM l-glutamine (HyClone, South Logan, UT, USA), 100 units/ml penicillin (HyClone) and 100 μg/ml streptomycin (HyClone) in a 37 °C incubator with 5% CO2.
Chen L.M., Yang P.P., Al Haq A.T., Hwang P.A., Lai Y.C., Weng Y.S., Chen M.A, & Hsu H.L. (2022). Oligo-Fucoidan supplementation enhances the effect of Olaparib on preventing metastasis and recurrence of triple-negative breast cancer in mice. Journal of Biomedical Science, 29, 70.
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9

Cell Line Maintenance and Transfection Protocol

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MDA-MB-231, BT-549, Hs578T, MDA-MB-468, MDA-MB-453, HCC1395, and HEK293 cells were purchased from ATCC (Manassas, VA, USA), SUM159PT were obtained from Dr. Joe Gray (OHSU) and HUVECs were purchased from EMD-Millipore (Billerica, MA, USA). All cell lines were validated by STR profiling and confirmed to be free of mycoplasma contamination. MDA-MB-231, Hs578T, MDA-MB-468, MDA-MB-453, and HEK293 cells were grown in high glucose DMEM (Gibco, Thermo Fisher, Waltham, MA, USA) supplemented with 10% FBS (Atlanta Biologicals, Flowery Branch, GA, USA). BT-549 and HCC1395 cells were grown in RPMI (Gibco) supplemented with 10% FBS. SUM159PT were grown in Hams-F12 media (Gibco) supplemented with 5% FBS, 10mM HEPES, 4μg/mL insulin, and 0.5μg/mL hydrocortisone. HUVECs were grown in complete EndoGRO medium (Millipore).
MiRIDIAN microRNA mimics as well as ON-TARGETplus SMARTpool and ON-TARGETplus individual siRNAs (listed in Supplementary Table 2) were purchased from GE Dharmacon (Lafayette, CO, USA), and transfected using Lipofectamine 2000 or Lipofectamine 3000 (Life Technologies, Carlsbad, CA, USA) according to manufacturer’s protocol at a final concentration of 25nM. BT-549 cells were transfected with LF2000 on two consecutive days to increase transfection efficiency. Phase images of cells were taken on an EVOS-FL scope (Thermo Fisher).
Langer E.M., Kendsersky N.D., Daniel C.J., Kuziel G.M., Pelz C., Murphy K.M., Capecchi M.R, & Sears R.C. (2017). ZEB1-repressed microRNAs inhibit autocrine signaling that promotes vascular mimicry of breast cancer cells. Oncogene, 37(8), 1005-1019.
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10

Breast Cancer Cell Line Responses to Guadecitabine and IFNy

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Human breast cancer cell lines MCF7, BT474, BT549, and HCC1395 were obtained from ATCC. MCF7 and BT474 were grown in DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS) (Denville). BT549 and HCC1395 were grown in RPMI (Gibco) supplemented with 10% FBS. The murine mammary carcinoma cell line, MMTV-Neu, was originally isolated from a primary mammary tumor (transgenic FVB/N mice) and cultured in DMEM/F12 (Gibco) supplemented with 10% FBS, 20 ng/ml EGF (Gibco), 0.5 µg/ml Hydrocortisone (Santa Cruz), and 10 µg/ml Insulin (Gibco). MCF7, BT474, BT549, HCC1395, and MMTV-Neu cells were pre-treated with diluent (Astex Pharmaceuticals), 0.05 µg/ml guadecitabine (Astex Pharmaceuticals) or 0.5 µg/ml guadecitabine for 3 days and stimulated with 100 ng/ml IFNγ (Gibco) for an additional 3 days with or without the presence of diluent control or guadecitabine. All cell lines were routinely tested for mycoplasm contamination.
Luo N., Nixon M.J., Gonzalez-Ericsson P.I., Sanchez V., Opalenik S.R., Li H., Zahnow C.A., Nickels M.L., Liu F., Tantawy M.N., Sanders M.E., Manning H.C, & Balko J.M. (2018). DNA methyltransferase inhibition upregulates MHC-I to potentiate cytotoxic T lymphocyte responses in breast cancer. Nature Communications, 9, 248.
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