β tubulin antibody

Cells were scraped into sodium dodecyl sulfate (SDS) loading buffer (40% glycerol, 0.24 M Tris-HCl, pH 6.8, 8% SDS, 0.04% bromophenol blue, 5% β-mercaptoethanol) and heated at 95°C for 5 min before being loaded onto SDS polyacrylamide gels for Western blot analysis. The following antibodies and dilutions were used: Flag antibody (MBL, Tokyo, Japan, M185-3L), 1:5,000; ICP0 antibody (Santa Cruz, 11060), 1:2,000; β-tubulin antibody (Sungene Biotech, Tianjin, China, KM9003), 1:5,000; rabbit HA antibody (Sangon, Shanghai, China, D110004), 1:1,000; β-actin antibody (Abclonal, Wuhan, China, Ac026), 1:5,000; ICP4 antibody (Abcam, Cambridge, England, ab6514), 1:5,000; gC antibody (Fitzgerald, North Acton, MA, United States, 10-H25A), 1:1,000; ICP27 antibody (Virusys, Taneytown, MD, United States, 1113), 1:5,000; ACOT8 rabbit polyclonal antibody (Sangon, D221492), 1:500; C1QBP rabbit polyclonal antibody (Sangon, D152901), 1:1,000; OTUD4 rabbit polyclonal antibody (Abclonal, A15229): 1:1,000; SNX9 rabbit polyclonal antibody (Sangon, D154017), 1:1,000; VIM rabbit polyclonal antibody (Sangon, D220268), 1:3,000; horseradish peroxidase (HRP)-conjugated goat anti-mouse (SouthernBiotech, Birmingham, AL, United States, 1030-05), 1:2,000; goat anti-rabbit antibodies (SouthernBiotech, 4030-05), 1:2,000.