The human NCI-H295R cell line, derived from a primary ACC in a female patient [44 (link)], was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (RRID:CVCL_0458) and cultured as indicated. The MUC-1 cell line, established from a neck metastasis of an EDP-M-treated male patient, was kindly donated by Dr. Hantel and cultured as suggested [45 (link)]. Additionally, the new ACC cell line TVBF-7 [46 (link)] was established from a primary culture derived from a perirenal lymph-node metastasis of a male ACC patient who underwent progression after EDP-M. A detailed description of these three cell lines was provided by Sigala et al. [47 (link)]. All three cell lines were periodically tested for mycoplasmas and authenticated by genetic profiling using polymorphic short tandem repeat loci with the PowerPlex Fusion system (Promega, BMR Genomics Cell Profile service, Padova, Italy).
Nci h295r cell line
Manufactured by American Type Culture Collection
Sourced in United States
The NCI-H295R cell line is a human adrenocortical carcinoma cell line. It is used for the study of adrenal steroidogenesis and the effects of various compounds on adrenal cell function.
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3 protocols using nci h295r cell line
1
Establishment and Characterization of ACC Cell Lines
2
Cell Line Cultivation Protocols
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The human NCI-H295R cell line, derived from a primitive ACC in a female patient (27 (link)), was obtained from the American Type Culture Collection (ATCC) and cultured as indicated by ATCC. MUC-1 cell line, established form a neck metastasis of an EDP-M treated male patient, was kindly given by Dr. Hantel and cultured as suggested (28 (link)). Media and supplements were supplied by Sigma Aldrich Italia, (Milan, Italy).
3
TUDCA Cytotoxicity Evaluation in SW-13 and NCI-H295R Cells
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The SW-13 cell line was obtained from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (cat. no. TCHu221). The NCI-H295R cell line was obtained from the American Type Culture Collection (cat. no. ATCC® CRL-2128). Cells were grown in minimum essential medium supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin solution (Beijing Solarbio Science and Technology Co., Ltd.). Cells were cultured at 37°C in a humidified atmosphere with 5% CO2 and 95% humidity in an incubator. TUDCA was purchased from EMD Millipore. SW-13 cells were treated with 0, 100, 200, 300 or 400 µM TUDCA, and NCI-H295R cells were treated with 0, 100, 200, 400 or 600 µM TUDCA.
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