The IUPAC name of CHT is: Tetrachloroisophthalonitrile (CAS no. 1897-45-6). The CHT (obtained from Labservices, Bologna, Italy) was first dissolved in dimethyl sulfoxide (DMSO) (CAS no. 67-68-5) at a final concentration of 0.6 mg/mL (stock solution) and was kept at 4 °C until prepared for the final exposure solutions in culture medium. Gibco RPMI 1640 cell culture media supplemented with L-glutamine, foetal calf serum, phytohemagglutinin (PHA), and antibiotics were purchased from Invitrogen-Life Technologies, Milan, Italy. Cytochalasin-B, colchicine and Mitomycin-C (MMC) were obtained from Sigma-Aldrich, Milan, Italy. Methanol, Acetic acid, Giemsa stain solution, and conventional microscope slides were purchased from Carlo Erba Reagenti, Milan, Italy. Potassium chloride (KCl) and Sörensen buffer were obtained from Merck S.p.A., Milan, Italy. Vacutainer blood collection tubes were from Terumo Europe, Rome, Italy. Distilled water was used throughout the experiments.
Vacutainer blood collection tubes
Manufactured by Terumo
Sourced in Italy
Vacutainer blood collection tubes are sterile, evacuated tubes designed for the collection of venous blood samples. They are used to collect and transport blood specimens for laboratory analysis.
Automatically generated - may contain errors
Lab products found in correlation
Mitomycin c, by Merck Group (3 mentions)
L glutamine, by Thermo Fisher Scientific (3 mentions)
Potassium chloride (kcl), by Merck Group (3 mentions)
Cytochalasin b, by Merck Group (3 mentions)
Phytohemagglutinin (pha), by Thermo Fisher Scientific (2 mentions)
Foetal calf serum, by Thermo Fisher Scientific (2 mentions)
S rensen buffer, by Merck Group (2 mentions)
Gibco rpmi 1640 cell culture media, by Thermo Fisher Scientific (2 mentions)
Methanol, by Carlo Erba (2 mentions)
Acetic acid, by Carlo Erba (2 mentions)
Conventional microscope slides, by Carlo Erba (2 mentions)
Giemsa stain solution, by Merck Group (2 mentions)
Colchicine, by Merck Group (1 mentions)
Giemsa, by Merck Group (1 mentions)
4 protocols using vacutainer blood collection tubes
1
Cytogenetic Analysis of CHT Exposure
2
Genotoxicity Assessment of Oxybenzone
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The IUPAC name of Oxybenzone (CAS n. 131-57-7) is 2-Hydroxy-4-methoxybenzophenone or BP-3.
The BP-3 was dissolved in dimethyl sulfoxide (DMSO, . Gibco RPMI 1640 cell culture media supplemented with L-glutamine, foetal calf serum, phytohemagglutinin (PHA), and antibiotics were purchased from Invitrogen-Life Technologies, Milan, Italy. Cytochalasin-B, Mitomycin-C (MMC), DMSO, BP-3 and Giemsa stain solution were obtained from Sigma-Aldrich, Milan, Italy. Methanol, Acetic acid, and conventional microscope slides were purchased from Carlo Erba Reagenti, Milan, Italy. Potassium chloride (KCl) and Sörensen buffer were obtained from Merck S.p.A., Milan, Italy. Vacutainer blood collection tubes were from Terumo Europe, Rome, Italy.
The BP-3 was dissolved in dimethyl sulfoxide (DMSO, . Gibco RPMI 1640 cell culture media supplemented with L-glutamine, foetal calf serum, phytohemagglutinin (PHA), and antibiotics were purchased from Invitrogen-Life Technologies, Milan, Italy. Cytochalasin-B, Mitomycin-C (MMC), DMSO, BP-3 and Giemsa stain solution were obtained from Sigma-Aldrich, Milan, Italy. Methanol, Acetic acid, and conventional microscope slides were purchased from Carlo Erba Reagenti, Milan, Italy. Potassium chloride (KCl) and Sörensen buffer were obtained from Merck S.p.A., Milan, Italy. Vacutainer blood collection tubes were from Terumo Europe, Rome, Italy.
3
Bisphenol A Cytogenetic Assay Protocol
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The IUPAC name of bisphenol A (BP-A, CAS n. 80-05-7) is 4,4 0 -(propane-2,2-diyl)diphenol. The BP-A was dissolved in dimethyl sulfoxide (DMSO, CAS no. 67-68-5). Gibco RPMI 1640 cell culture media supplemented with L-glutamine, foetal calf serum (FCS), phytohaemagglutinin (PHA) and antibiotics were purchased from Invitrogen-Life Technologies, Milan, Italy. Cytochalasin-B, mitomycin-C (MMC), BP-A and Giemsa stain solution were obtained from Sigma-Aldrich, Milan, Italy. Methanol, acetic acid and conventional microscope slides were purchased from Carlo Erba Reagenti, Milan, Italy. Potassium chloride (KCl) and So ¨rensen buffer were obtained from Merck S.p.A., Milan, Italy. Vacutainer blood collection tubes were from Terumo Europe, Rome, Italy.
4
Oxybenzone Stability and Photodegradation
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The IUPAC name of Oxybenzone (CAS n. 131-57-7) is 2-Hydroxy-4-methoxybenzophenone or BP-3.
BP-3 and Giemsa stain solution were obtained from Sigma-Aldrich, Milan, Italy. Methanol, acetic acid, and conventional microscope slides were purchased from Carlo Erba Reagenti, Milan, Italy. Potassium chloride (KCl) and Sörensen buffer were obtained from Merck S.p.A., Milan, Italy. Vacutainer blood collection tubes were from Terumo Europe, Rome, Italy.
As for its stability and photodegradation, BP-3 was found to degrade only about 4% after 28 days in water and to remain persistent after 24 h of simulated sunlight irradiation [43 (link)].
The chemistry of the tap water used for L. stagnalis experiments was as follows: pH: 7.3; dry residue at 180 °C: 313 mg/L; calcium: 71 mg/L; magnesium: 13 mg/L; ammonium: <0.05 mg/L; chlorides: 17 mg/L; sulfates: 35 mg/L; potassium: 2 mg/L; sodium: <10 mg/L; arsenic: <1 mg/L; bicarbonates: 238 mg/L; free residual chlorine: 0.1 mg/L; fluorides: <0.1 mg/L; nitrates: 21 mg/L; nitrites: <0.05 mg/L, and manganese: <1 µg/L. The BP-3 was not found [44 ].
The artificial seawater used for the experiments on O. diadema was made in the laboratory starting from deionized water, to which the necessary quantity of salts was added to reach the final salinity of 35‰.
BP-3 and Giemsa stain solution were obtained from Sigma-Aldrich, Milan, Italy. Methanol, acetic acid, and conventional microscope slides were purchased from Carlo Erba Reagenti, Milan, Italy. Potassium chloride (KCl) and Sörensen buffer were obtained from Merck S.p.A., Milan, Italy. Vacutainer blood collection tubes were from Terumo Europe, Rome, Italy.
As for its stability and photodegradation, BP-3 was found to degrade only about 4% after 28 days in water and to remain persistent after 24 h of simulated sunlight irradiation [43 (link)].
The chemistry of the tap water used for L. stagnalis experiments was as follows: pH: 7.3; dry residue at 180 °C: 313 mg/L; calcium: 71 mg/L; magnesium: 13 mg/L; ammonium: <0.05 mg/L; chlorides: 17 mg/L; sulfates: 35 mg/L; potassium: 2 mg/L; sodium: <10 mg/L; arsenic: <1 mg/L; bicarbonates: 238 mg/L; free residual chlorine: 0.1 mg/L; fluorides: <0.1 mg/L; nitrates: 21 mg/L; nitrites: <0.05 mg/L, and manganese: <1 µg/L. The BP-3 was not found [44 ].
The artificial seawater used for the experiments on O. diadema was made in the laboratory starting from deionized water, to which the necessary quantity of salts was added to reach the final salinity of 35‰.
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