Cary series 2

Electronic absorption spectra of NTBD were recorded on a double-beam UV–Vis spectrophotometer Cary 300 Bio (Varian) equipped with a thermostated tray holder with a 6 × 6 multi-cell Peltier block. The temperature was controlled with a thermocouple probe (Cary Series II from Varian) placed directly in the sample.
Cary Eclipse spectrofluorometer (Varian) was applied for recording fluorescence excitation, emission and synchronous spectra. All measurements were carried out at 22 °C. All the fluorescence spectra were recorded with 0.5 nm resolution together with the lamp and photomultiplier spectral characteristics corrections. Resonance light scattering (RLS) measurements were carried out according to the previously reported protocol with synchronous scanning of both the excitation and emission monochromators (there was no interval between excitation and emission wavelengths) and spectral resolution of 1.5 nm. Grams/AI 8.0 software (Thermo Electron Corporation; Waltham, Massachusetts, United States) was applied for an analysis of the recorded data.

The UV-Vis spectra were recorded using a Cary 300 Bio (Varian, Santa Clara, CA, USA) spectrophotometer with a tray holder thermostatted by 6 × 6 multi-cell Peltier block. A thermocouple probe (Cary Series II from Varian) placed directly in the sample was used for temperature control.
A Cary Eclipse spectrofluorometer (Varian) was used for fluorescence excitation, emission and synchronous spectra measurements at 22 °C. Fluorescence spectra were recorded at the resolution of 0.5 nm and corrected for the spectral characteristics of the lamp and photomultiplier. Resonance light scattering (RLS) measurements were recorded according to the previously reported protocol [54 (link)]. The excitation and emission monochromators of the spectrofluorometer were scanned synchronously (0.0 nm interval between the excitation and emission wavelengths); the slits were set to obtain a spectral resolution of 1.5 nm. Grams/AI 8.0 software (Thermo Electron Corporation, Waltham, MA, USA) was applied for the analysis of spectroscopic data.

Electronic absorption spectra of C1 and C7 were recorded on a double-beam UV-Vis spectrophotometer Cary 300 Bio (Varian) equipped with a thermostatted cuvette holder with a 6 × 6 multicell Peltier block. Temperature was controlled with a thermocouple probe (Cary Series II from Varian) placed directly in the sample. All experiments were carried out at 23 °C. The spectral slit width was 1.5 nm in the measurements of the electron absorption spectra.