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Ncr nude mice

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The NCr nude mice are a strain of genetically modified mice that lack a functional thymus gland, resulting in a deficiency of T cells. This makes them immunodeficient and suitable for use in various research applications where the study of human cells or tissues in a living organism is required, such as xenograft studies.

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Lab products found in correlation

Rbc lysis buffer, by Qiagen (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Matrigel, by Corning (1 mentions) Saline matrigel, by BD (1 mentions)

7 protocols using ncr nude mice

1

Xenograft Tumor Treatment Study

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RCC xenograft tumors were established by SQ injection of 786-O cells into NCr nude mice (Charles River, Wilmington, MA, USA). When tumors reached approximately 1.5 cm in diameter, tumors were sterilely excised and 2 mm fragments used to inoculate naïve NCr nude mice (n=8/group). For treatment studies, mice were entered onto treatment when tumor volumes reached 200 mm3. Mice were treated with daily i.p. injections of amitriptyline 10 mg/kg, oxaprozin 20 mg/kg, or pentamidine 20 mg/kg or PBS (vehicle). The statistical significance of drug treatments was determined by 2-way ANOVA.
Zerbini L.F., Bhasin M.K., de Vasconcellos J.F., Paccez J.D., Gu X., Kung A.L, & Libermann T.A. (2014). Computational repositioning and preclinical validation of pentamidine for renal cell cancer. Molecular cancer therapeutics, 13(7), 1929-1941.
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2

In Vivo Bioluminescent Imaging of Immune Cells

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All animal procedures and imaging protocols are performed under DFCI institutional IACUC guidelines. Splenocytes were harvested from NCr nude mice (Charles River Laboratories, Wilmington MA) and were treated with RBC lysis buffer (Qiagen, Valencia CA). The cells were then washed with HBSS prior to a 24 h culture period in RPMI 1640 containing 10 % FCS and 5 mg/mL of lipopolysaccharide (LPS, from Samonella enterica, Sigma Aldrich). The cells were collected and then stained with HBSS containing 1 μM of DiR. To generate light signal, the DiR-stained splenocytes suspension (100 μL, 1x107) was transferred into 100 μL of HBSS containing 2 μL of CSPD alkaline phosphatase substrate (Applied Biosystems) on a black 96-well plate. Untreated splenocytes cells were used as negative controls. For in vivo imaging, NCr nude mice received i.p. injection of 10 mg/kg LPS (Sigma Aldrich) in normal saline. In some animals, dexamethasone sodium phosphate (10 mg/kg, American Regent Inc., Shirley NY) was given 1 h prior to LPS challenge. 24 h later, the animal received an i.p. injection of 400 μL solution containing 50 % CSPD, 50 % reaction diluent, and 0.25 mg/mL DiR, prior to imaging. Cell and animal luminescence were scanned from 520 to 800 nm in 40 nm steps.
Tseng J.C, & Kung A.L. (2015). In vivo imaging of endogenous enzyme activities using luminescent 1,2-dioxetane compounds. Journal of Biomedical Science, 22(1), 45.
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3

Tumorigenicity Assay in Nude Mice

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To measure in vivo tumorigenicity, engineered NIH3T3 and SW480 cells expressing WT or the mutant form of UVRAG (5 × 106) were transplanted into the flanks of six-week-old female NCR nude mice (Charles River). Ten mice per cell line were used. Mice were monitored tri-weekly for the development of tumors, and necropsied after a three-week observation period. The tumor growth was monitored by measurements of tumor length (L) and width (W) and tumor volume was calculated49 (link) using the following formula: Volume = 4/3 × π × (1/2 width)2 × 1/2 length. All animal studies were performed in compliance with the University of Southern California Institutional Animal Care and Use Committee (IACUC) guidelines.
He S., Zhao Z., Yang Y., O'Connell D., Zhang X., Oh S., Ma B., Lee J.H., Zhang T., Varghese B., Yip J., Dolatshahi Pirooz S., Li M., Zhang Y., Li G.M., Ellen Martin S., Machida K, & Liang C. (2015). Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers. Nature Communications, 6, 7839.
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4

Tumor Modeling and Drug Efficacy

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Animal experiments were carried out according to IACUC-approved procedures at the University of California San Diego. MDA-MB-231 cells were resuspended to a concentration of 1 × 106 cells per mL in a 1:1 ratio of RPMI medium and Matrigel (Corning Life Sciences, New York, NY, USA). We injected 100 μL of the cell suspension subcutaneously into the right hind leg of NCr nude mice (Charles River, Wilmington, MA, USA) at 4–6 weeks of age. The mice were treated with drugs or VLPs when the tumor volume reached ~150 mm3 (~11 days postinjection). Treatment was administered intravenously twice weekly at a dosage of 1.0 mg/kg body weight normalized to Pt. Five independent groups of n = 10 animals were established (PBS, PhMVCy5.5-PEG, cisplatin, Pt-Mal, and Pt-PhMVCy5.5-PEG). Tumor size and body weight were measured before each injection and the volume was calculated using the formula ν = l × w2/2. Mice were euthanized if the tumor size exceeded 1000 mm3 according to IACUC guidelines. For biodistribution, mice (n = 3) were euthanized after three intravenous doses of cisplatin, Pt-Mal, or Pt-PhMVCy5.5-PEG in 24 h. Organs were collected and the Pt content was determined by ICP-MS, as described above.
Hu H, & Steinmetz N.F. (2020). Cisplatin Prodrug-Loaded Nanoparticles Based on Physalis Mottle Virus for Cancer Therapy. Molecular pharmaceutics, 17(12), 4629-4636.
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5

In Vivo Tumorigenicity Assay

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To measure in vivo tumorigenicity, engineered NIH3T3 and SW480 cells expressing WT or the mutant form of UVRAG (5 × 106) were transplanted into the flanks of six-week-old female NCR nude mice (Charles River). Ten mice per cell line were used. Mice were monitored triweekly for the development of tumours, and necropsied after a 3-week observation period. The tumour growth was monitored by measurements of tumour length (L) and width (W) and tumour volume was calculated47 (link) using the following formula: Volume=4/3 × π × (1/2 width)2 × 1/2 length. All animal studies were performed in compliance with the University of Southern California Institutional Animal Care and Use Committee guidelines.
He S., Zhao Z., Yang Y., O'Connell D., Zhang X., Oh S., Ma B., Lee J.H., Zhang T., Varghese B., Yip J., Dolatshahi Pirooz S., Li M., Zhang Y., Li G.M., Ellen Martin S., Machida K, & Liang C. (2015). Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers. Nature Communications, 6, 7839.
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6

Subcutaneous Xenografts and Brain Metastasis

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Six to eight weeks-aged female mice were used for animal studies. NCR nude mice, purchased from Charles River Laboratories (Wilmington, MA), were used for subcutaneous xenografts studies. NSG mice were used for brain metastasis study. All animal experiments were performed by following MGH and MDACC Animal Care and Use Committee guidelines.
Yuan P., Teng D., de Groot E., Li M., Trousil S., Shen C.H., Roszik J., Davies M.A., Gopal Y.N, & Zheng B. (2023). Loss of AMPKα2 promotes melanoma tumor growth and brain metastasis. iScience, 26(6), 106791.
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7

Xenograft Mouse Model of ABC294640

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All procedures involving mice were performed in accordance with Pennsylvania State University IACUC protocols. 7 week old male NCR-nude mice (Charles Rivers) were subcutaneously injected in the flank with 3×106 cells in 100 μl total saline/Matrigel (BD Biosciences). When tumors reached ~100–150 mm3 mice were administered 50mg/kg ABC294640 or Vehicle (46% PEG400/47% Saline/7% EtOH) daily. Tumor volume was measured with calipers.
Schrecengost R.S., Keller S.N., Schiewer M.J., Knudsen K.E, & Smith C.D. (2015). Downregulation of Critical Oncogenes by the Selective SK2 Inhibitor ABC294640 Hinders Prostate Cancer Progression. Molecular cancer research : MCR, 13(12), 1591-1601.
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