Primary human umbilical vein endothelial cells (HUVECs) were purchased from Cascade Biologics. FN, MGO and su6656 were obtained from Sigma. Recombinant VEGF‐A, recombinant RAGE, recombinant human integrin α5β1 protein, RAGE function‐blocking antibody, biotinylated anti‐RAGE antibody, biotinylated anti‐integrin β1 antibody, streptavidin‐HRP conjugate and HRP substrate were from R&D Systems. Growth‐factor‐reduced Matrigel Matrix was from BD Biosciences. Antibodies to phosphorylated VEGFR‐2, total VEGFR‐2, phosphorylated Akt, total Akt, phosphorylated extracellular regulated protein kinases 1/2 (ERK1/2), total ERK1/2, phosphorylated nuclear factor‐κB (NF‐κB), total NF‐κB and CD31 were from Cell Signaling Technology. Antibodies to FN and AGEs were from Abcam. Antibodies to RAGE and c‐Src were from Santa Cruz Biotechnology. Pierce classic IP kit was from Thermo Scientific.
Primary human umbilical vein endothelial cells huvecs
Manufactured by Thermo Fisher Scientific
Sourced in United States
Primary human umbilical vein endothelial cells (HUVECs) are a type of cell derived from the human umbilical vein. They are used in cell culture and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Pierce classic ip kit, by Thermo Fisher Scientific (1 mentions)
Su6656, by Merck Group (1 mentions)
Recombinant vegfa, by R&D Systems (1 mentions)
Streptavidin hrp conjugate, by R&D Systems (1 mentions)
Hrp substrate, by R&D Systems (1 mentions)
Matrigel matrix growth factor reduced, by BD (1 mentions)
Total nf κb p65, by Cell Signaling Technology (1 mentions)
Medium 200, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8 solution, by Beyotime (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Bay 11 7082, by Beyotime (1 mentions)
2 protocols using primary human umbilical vein endothelial cells huvecs
1
Exploring VEGFR-2 and Integrin Signaling in HUVECs
2
HUVEC Inflammatory Response Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary human umbilical vein endothelial cells (HUVECs) and Medium 200 were purchased from Cascade Biologics (Portland, OR, USA). STS (purity ≥98%) was from National Institutes for Food and Drug Control (Beijing, China).
LPS was purchased from Sigma (St Louis, MO, USA). Antibodies to TNF-α, IL-1β, phosphorylated NF-κB p65, total NF-κB p65, phosphorylated IκBα, total IκBα and GAPDH were form Cell Signaling Technology (Beverly, MA, USA).
Fluorescein isothiocyanate (FITC)-conjugated anti-rabbit IgG was obtained from Molecular Probes (Eugene, OR, USA). Human IL-1β and TNF-α D r a f t enzyme-linked immunosorbent assay (ELISA) kits were from R&D Systems (Minneapolis, MN, USA). Cell Counting Kit 8 (CCK8) solution and NF-κB inhibitor, BAY11-7082 were from Beyotime (Shanghai, China). STS was dissolved in phosphate buffer saline (PBS) to obtain a 1 mg/mL stock solution.
LPS was purchased from Sigma (St Louis, MO, USA). Antibodies to TNF-α, IL-1β, phosphorylated NF-κB p65, total NF-κB p65, phosphorylated IκBα, total IκBα and GAPDH were form Cell Signaling Technology (Beverly, MA, USA).
Fluorescein isothiocyanate (FITC)-conjugated anti-rabbit IgG was obtained from Molecular Probes (Eugene, OR, USA). Human IL-1β and TNF-α D r a f t enzyme-linked immunosorbent assay (ELISA) kits were from R&D Systems (Minneapolis, MN, USA). Cell Counting Kit 8 (CCK8) solution and NF-κB inhibitor, BAY11-7082 were from Beyotime (Shanghai, China). STS was dissolved in phosphate buffer saline (PBS) to obtain a 1 mg/mL stock solution.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!