1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] (DSPE-PEG2000-COOH) was purchased from Ponsure Biotechnology (Shanghai, China). Calcium chloride (CaCl2) and trisodium phosphate (Na3PO4) were purchased from Sigma-Aldrich (USA). BAF312 was purchased from Selleck (Shanghai, China). BAF312-loaded DSPE-PEG2000 micelles were made through thin-membrane hydration. Then, CaCl2 solution was added to the prepared micelle solution. Finally, HBS (Hepes, Na3PO4, and NaCl; pH 7.4) buffer solution was added into the BAF312-DSPE-PEG2000-Ca2+ solution quickly and allowed to incubate at room temperature for 30 min to form BAF312@CaP-NPs. The final concentration of BAF312 was 0.25 μM. To meet the demands of subsequent experiments, during the preparation of NPs, BAF312 was replaced by rhodamine B (Rb) (Beyotime, China) to prepare Rb@CaP-NPs.
Trisodium phosphate na3po4
Manufactured by Merck Group
Sourced in United States, Germany
Trisodium phosphate (Na3PO4) is an inorganic compound used as a lab equipment product. It is a white, crystalline solid that is soluble in water. Trisodium phosphate serves as a buffering agent, emulsifier, and cleaning agent in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Baf312, by Beyotime (1 mentions)
Baf312, by Selleck Chemicals (1 mentions)
Rhodamine b, by Beyotime (1 mentions)
Salicylaldehyde, by Merck Group (1 mentions)
Cyclohexanone, by Merck Group (1 mentions)
Muse cell cycle kit, by Merck Group (1 mentions)
4 hydroxy 3 methoxybenzaldehyde, by Merck Group (1 mentions)
2 hydroxy 4 methoxybenzaldehyde, by Merck Group (1 mentions)
Muse system check kit, by Merck Group (1 mentions)
Muse count viability kit, by Merck Group (1 mentions)
Boric acid h3bo3, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
4 hydroxybenzaldehyde, by Merck Group (1 mentions)
2 protocols using trisodium phosphate na3po4
1
Synthesis of Dual-Functional Calcium Phosphate Nanoparticles
2
Cytotoxicity Screening of Benzaldehyde Derivatives
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4-Hydroxy-3-methoxybenzaldehyde, 2-hydroxy-4-methoxybenzaldehyde (98%), salicylaldehyde (98%), 4-hydroxy-benzaldehyde (98%), cyclohexanone (99%), boric acid (H3BO3, >99.5%), citric acid (>99.5%) trisodium phosphate (Na3PO4, 96%) and sodium hydroxide (NaOH, 99%) were acquired from Sigma Aldrich (Steinheim am Albuch, Germany). Methanol (MeOH, >99.9%) and sulphuric acid (H2SO4, 95–97%) were purchased from CHIMREACTIV SRL (Bucuresti, Romania) and Merck KGaA (Darmstadt, Germany). All reagents and solvents used in the experiments were used without further purification.
Fetal bovine serum (FBS), Dulbecco’s phosphate-buffered saline (DPBS without Ca and Mg), Trypsin, Minimum Essential Medium α (α-Mem), Roswell Park Memorial Institute (RPMI) 1640 Medium with Glutamax, and Penicillin-Streptomycin (Penstrep) were acquired from Gibco ThermoScientific LSG (Auckland, New Zealand). Dimethylsulfoxide was purchased from Fluka Analytical (Seelze, Germany). Muse System Check Kit, Muse Count & Viability Kit and Muse Cell Cycle Kit were acquired from Merck KGaA (Darmstadt, Germany).
Fetal bovine serum (FBS), Dulbecco’s phosphate-buffered saline (DPBS without Ca and Mg), Trypsin, Minimum Essential Medium α (α-Mem), Roswell Park Memorial Institute (RPMI) 1640 Medium with Glutamax, and Penicillin-Streptomycin (Penstrep) were acquired from Gibco ThermoScientific LSG (Auckland, New Zealand). Dimethylsulfoxide was purchased from Fluka Analytical (Seelze, Germany). Muse System Check Kit, Muse Count & Viability Kit and Muse Cell Cycle Kit were acquired from Merck KGaA (Darmstadt, Germany).
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