Annexin buffer
Annexin buffer is a solution used to maintain the structural integrity and functional properties of annexin proteins. It provides a controlled environment for annexin-based assays and experiments.
Lab products found in correlation
9 protocols using annexin buffer
Annexin V-FITC Apoptosis Assay
Evaluation of Cisplatin, Cetuximab, and Panitumumab Efficacy
Radiation-Induced Apoptosis in CD34+ Cells
Cell Cycle and Apoptosis Analysis in MSTO-211H Cells
For cell cycle analysis after CDKi treatment, 2 × 105 MSTO-211H cells and 1.5 × 105 NCI-H2052 cells were seeded in Matrigel-coated 12-well plates (Corning). 16h later, CDK9i was added and after 12 h cells were synchronized and processed as described above.
Comprehensive Immune Cell Profiling
Platelet Extracellular Vesicle Characterization
Flow Cytometry of Cell Apoptosis
Apoptosis and Necrosis Quantification in U-87 Cells
of U-87 was measured 4 h after X-ray exposure by flow cytometry. X-ray-exposed
unstained and NT-PEO-Por*-stained U-87 were detached from dishes by
0.25% trypsin–EDTA, centrifuged at 1200 rpm for 5 min, and
resuspended in 100 μL of the annexin buffer (BD) and 5 μL
of Annexin V BV421 (BD), which binds to phosphatidylserin (PS) residues
and allows the identification of apoptotic cells. Cells were incubated
a 4 °C for 20 min, washed in the annexin buffer, and centrifuged.
The viability dye 7-aminoactinomycin D (7-AAD, BD) was added to cell
pellets to stain non-viable cells and recognize late-apoptotic fractions.
For fluorescence-activated cell sorting (FACS) characterization, data
were obtained using a FACSAria Fusion cell sorter, equipped with five
lasers, and analyzed with FACSDiva software (ver. 8.0, BD). At least
10 × 103 events were recorded for each condition.
Debris events were excluded from the analysis by morphological gating
(side scatter vs forward scatter dot plot).
Flow Cytometric Analysis of Apoptosis and Cell Cycle in Thymic Cells
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