Cd90.2 53 2 1

Manufactured by BD

Sourced in Canada

CD90.2 (53–2.1) is a laboratory reagent used for the identification and quantification of cells expressing the CD90.2 (Thy-1.2) antigen. This antigen is commonly expressed on various cell types, including T cells, neurons, and certain stem cells. The reagent can be used in flow cytometry and other immunoassays to detect and analyze CD90.2-positive cells.

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Lab products found in correlation

Lsrii flow cytometer, by BD (2 mentions) Ic fixation buffer, by Thermo Fisher Scientific (2 mentions) Fixable aqua dead cell staining, by Thermo Fisher Scientific (2 mentions) Cd11b clone rm2817, by Thermo Fisher Scientific (2 mentions) Cd127 clone a7r34, by BD (2 mentions) Cd45 clone 30 f11, by Thermo Fisher Scientific (2 mentions) Ly6c clone al 21, by BD (2 mentions) Cd19 clone 1d3, by BD (2 mentions) Cd8b clone yts156, by BioLegend (2 mentions) Cd3ε clone 145 2c11, by BD (2 mentions) Facsaria 2, by BD (1 mentions) Alexa fluor 647 goat anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions) Facscalibur, by BD (1 mentions) Efluor 780, by Thermo Fisher Scientific (1 mentions) Facscelesta, by BD (1 mentions) Facsaria fusion, by BD (1 mentions) Foxp3 fjk 16s, by Thermo Fisher Scientific (1 mentions) Fcs express, by De Novo Software (1 mentions) Fc block 2.4g2, by BioXCell (1 mentions) Anti cd28 37, by BioXCell (1 mentions)

5 protocols using cd90.2 53 2 1

Comprehensive Immune Cell Profiling

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Antibodies against the following proteins were purchased from BD Biosciences: CD4 (RM4-5), CD69 (H1.2F3), CD90.1 (OX-7), phospho-STAT5^Y694 (C71E5), and CD90.2 (53-2.1). Antibodies against the following proteins were purchased from eBioscience: CD44 (IM7), CD98 (RL388), ICOS (7E.17G9), IRF4 (3E4), Ki-67 (SolA15), CD39 (24DMS1), KLRG1 (2F1), and FoxP3 (FJK-16s). Antibodies against the following proteins were purchased from BioLegend: CD4 (RM4-5), CD45 (30-F11), CD62L (MEL-14), CTLA-4 (UC10-4F10-11), PD-1 (29F.1A12), CD25 (PC61), and Bcl2 (BCL/10C4). Normal rabbit IgG (2729) and anti-phospho-S6^S240/244 (5364) were purchased from Cell Signaling Technology. Goat anti-rabbit-Alexa Fluor 647 secondary antibody was purchased from Invitrogen. Fc Block (2.4G2) and anti-CD28 (37.51) were purchased from Bio X Cell. Stimulatory anti-CD3 (2C11) was purified from hybridoma supernatants prepared in-house. Fixable viability dye eFluor780 was purchased from eBioscience. MitoTracker Deep Red dye was purchased from Invitrogen. Flow cytometry experiments were performed on a FACSCalibur, LSR II, or FACSCelesta (BD Biosciences), and analyzed using FlowJo software (Treestar, v.10.3) or FCS Express (De Novo Software, v.6). Cell sorting was performed on a FACSAria II or FACSAria Fusion (BD Biosciences).

Sun I.H., Oh M.H., Zhao L., C.h.i.r.a.g., Patel H., Arwood M.L., Xu W., Tam A.J., Blosser R.L., Wen J, & Powell J.D. (2018). mTORC1 signaling regulates the generation and function of central and effector FoxP3+ regulatory T cells : mTORC1 signaling controls central and effector Tregs. Journal of immunology (Baltimore, Md. : 1950), 201(2), 481-492.

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Flow Cytometry Immunophenotyping Panel

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TLR9 (J15A7; BD Biosciences; San Jose, CA), IgG1 (MOPC-21; BD Biosciences; San Jose, CA), CD45 (30-F11; BD Biosciences; San Jose, CA), CD11b (M1/70; BD Biosciences; San Jose, CA), CD11c (N418; Biolegend; San Diego, CA), Siglec F (E50-2440; BD Biosciences; San Jose, CA), MHC II (I-A/I-E) (M5/114.15.2; BD Biosciences; San Jose, CA), CD64 (X54-5/7.1; Biolegend; San Diego, CA), F4/80 (BM8 eBiosciences; SanDiego, CA), LY6G (1A8; BD Biosciences; San Jose, CA), CD3 (17A2, BD Biosciences; San Jose, CA), CD90.2 (53–2.1; BD Biosciences; San Jose, CA), CD4 (GK1.5; Biolegend; San Diego, CA) CD8 (53–6.7; BD Biosciences; San Jose, CA), NKP46 (29A1.4; Biolegend; San Diego, CA), CD19 (1D3; BD Biosciences; San Jose, CA), Fc Block(CD16/CD32) (2.4G2; BD Biosciences; San Jose, CA). The NP antibody used was MA1-7322 from Thermofisher (Waltham, MA) conjugated to FITC.

Martínez-Colón G.J., Warheit-Niemi H., Gurczynski S.J., Taylor Q.M., Wilke C.A., Podsiad A.B., Crespo J., Bhan U, & Moore B.B. (2019). Influenza-induced immune suppression to methicillin-resistant Staphylococcus aureus is mediated by TLR9. PLoS Pathogens, 15(1), e1007560.

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Multiparametric Immune Cell Profiling

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Lymphocytes were counted and subjected to viability staining (Fixable Aqua Dead Cell staining, Life Technologies, #L34957) and subsequently to receptor Fc blockade (BD #553142). Staining was performed using the following antibodies: CD4 (clone RM4-4, BD), CD45 (clone 30-F11 eBioscience), CD45.1 (clone A20, BD), CD45.2 (clone 104, BD) CD8a (clone 53-6.7, eBioscience or BD), CD8b (clone YTS156.7.7, BioLegend), CD11b (clone RM2817, Thermo Fisher), Ly6c (clone AL-21, BD), CD3ε (clone 145-2C11, BD), CD19 (clone 1D3, BD), CD90.2 (53-2.1, BD), CD127 (clone A7R34, BD), CX3CR1 (clone SA011F11, BioLegend), CXCR3 (clone CXCR3-173, Biolegend), CD103 (clone 2E7, eBioscience or Biolegend), CD69 (clone H1.2F3, BD), TCR-β (clone H57-597, BD, TCR-γδ (clone eBio-GL3, eBioscience). H2-K^b:SIINFEKL or H2-M3:fMIGWII tetramers were either produced in house or, for the former, obtained thorugh the NIH tetramer core. Samples were fixed (IC Fixation Buffer, eBioscience), washed, resuspended in FACS buffer and acquired with a LSRII flow cytometer (BD) either immediately or on the following day. For intracellular staining, the following antibodies were used: IFN-ϒ (clone XMG1.2, BD), TNF-α (clone MP6-XT22, BD) in Permeabilization buffer (eBioscience).

Becattini S., Littmann E.R., Seok R., Amoretti L., Fontana E., Wright R., Gjonbalaj M., Leiner I.M., Plitas G., Hohl T.M, & Pamer E.G. (2020). Enhancing mucosal immunity by transient microbiota depletion. Nature Communications, 11, 4475.

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Blood Cell Isolation and Phenotyping

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Blood was obtained by tail bleeding. Red blood cell lysis was performed by three consecutive incubations in RBC lysis buffer (0.15 M NH₄Cl = 1 mM NaHCO₃ in dH₂O) for 5′. Lymphocytes were counted and subjected to viability staining (Fixable Aqua Dead Cell staining, Life Technologies, #L34957) and subsequently to receptor Fc blockade (BD). Staining was performed using the following antibodies: CD45 (clone 30-F11; eBioscience), CD8b (clone YTS156.7.7; BioLegend), CD11b (clone RM2817; Thermo Fisher Scientific), Ly6c (clone AL-21; BD), CD3ε (clone 145-2C11; BD), CD19 (clone 1D3; BD), CD90.2 (53–2.1; BD), CD127 (clone A7R34; BD). Samples were fixed (IC Fixation Buffer; eBioscience), washed, resuspended in FACS buffer, and acquired with a LSRII flow cytometer (BD) either immediately or on the next day.

Becattini S., Littmann E.R., Carter R.A., Kim S.G., Morjaria S.M., Ling L., Gyaltshen Y., Fontana E., Taur Y., Leiner I.M, & Pamer E.G. (2017). Commensal microbes provide first line defense against Listeria monocytogenes infection. The Journal of Experimental Medicine, 214(7), 1973-1989.

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Murine Allergy Model Reagents

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Recombinant mouse IL-33 was purchased from Hokudo Co., Ltd (Sapporo, Japan). Fluorescent-labeled antibodies for CCR3 (83101) were purchased from R&D Systems (Minneapolis, MN). Antibodies for Siglec-F (E50-2440), CD45R/B220 (RA3-6B2), CD49b (DX5), CD11b (M1/70), CD11c (N418), and CD90.2 (53-2.1) were purchased from BD Biosciences (San Diego, CA). CD45 (30-F11), CD19 (6D5), CD3ε (145-2C11), IL-5 (TRFK5), CD16/32 (93), and streptavidin were purchased from BioLegend (San Diego, CA). Antibodies for IL-13 (eBio13A) and IgG1 isotype control (eBRG1) were purchased from eBioscience (San Diego, CA). Anti-IgE (23G3) was purchased from Southern Biotechnology Associates, Inc (Birmingham, AL). Biotinylated anti-T1/ST2 antibody was purchased from MD Biosciences (St Paul, MN). Ragweed pollen was purchased from PolyScience (Niles, IL). Ragweed extract was purchased from LSL Co Ltd (Tokyo, Japan). Anti–2,4-dinitrophenyl (DNP) IgE mAb (SPE-7) was purchased from Sigma-Aldrich (St Louis, MO). Purified mouse IgG1 λ isotype control was purchased from BD Biosciences.

Kato Y., Akasaki S., Muto-Haenuki Y., Fujieda S., Matsushita K, & Yoshimoto T. (2014). Nasal Sensitization with Ragweed Pollen Induces Local-Allergic-Rhinitis-Like Symptoms in Mice. PLoS ONE, 9(8), e103540.

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