Ab6673

Manufactured by BD

Ab6673 is a laboratory equipment designed for use in scientific research and analysis. It provides a core function of facilitating the detection and measurement of specific biological molecules or analytes. The detailed specifications and intended use of this product are not available in this response.

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Lab products found in correlation

Selenbp1, by Abcam (1 mentions) Lsm 710 confocal microscope, by Zeiss (1 mentions) Slowfade diamond antifade mountant, by Thermo Fisher Scientific (1 mentions) Ab3786, by Merck Group (1 mentions) Gfp 1020, by Abcam (1 mentions) Ddx0191p 100, by Abcam (1 mentions)

2 protocols using ab6673

Immunohistochemical Analysis of Tumor Samples

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Tumor samples were fixed in 4% formalin and paraffin embedded. Hematoxylin and Eosin staining was performed using standard methods. Images were quantified using ImageJ. For IHC, we used antibodies to Nfib (1:1000, Abcam ab186738), Uchl1 (1:500, sigma HPA005993), CC10 (CCSP) (1:1000, Millipore 07–623), Selenbp1 (1:200, Abcam), GFP (1:500, Abcam ab6673), Ascl1 (1:200, BD Biosciences, 556604) and RFP (1:500, Rockland).

Yang D., Denny S.K., Greenside P.G., Chaikovsky A.C., Brady J.J., Ouadah Y., Granja J.M., Jahchan N.S., Lim J.S., Kwok S., Kong C.S., Berghoff A.S., Schmitt A., Reinhardt H.C., Park K.S., Preusser M., Kundaje A., Greenleaf W.J., Sage J, & Winslow M.M. (2018). Inter-tumoral heterogeneity in SCLC is influenced by the cell-type of origin. Cancer discovery, 8(10), 1316-1331.

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Comprehensive Immunohistochemistry and RNA FISH Protocol

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IHC was performed with the following antibodies: Cleaved Caspase 3 (rabbit, R&D Systems, MAB835, 1:100), HOPX (mouse, Santa Cruz, sc-398703 1:00), GFP (chicken, Aves Labs, GFP-1020, 1:200 or goat, Abcam, ab6673, 1:200), Ki67 (mouse, BD Biosciences, 550609, 1:200), Lamp3 (DC-Lamp) (rat, Novus, DDX0191P-100, 1:100), NKX2.1 (Ttf1) (rabbit, Abcam, ab76013, 1:50), and SFTPC, (rabbit, Millipore Sigma, AB3786, 1:100). Slides were mounted using Slowfade Diamond Antifade Mountant (Invitrogen, catalog # S36972). RNA fluorescence in situ hybridization (FISH) was performed with RNAscope according the manufacturer’s instructions with the following probes: Mm-Col4a4-C2 (no. 1078041-C2), mm-Col4a3 (no. 544361), mm-Hopx-C2 (no. 405161-C2), mm-Igfbp7 (no. 425741), Mm-Lamb3 (no. 552161), and mm-Sftpc-C2 (no. 314101-C2). Fluorescent imaging was performed on a Zeiss LSM 710 confocal microscope with a 40 × water-immersion objective and processed using Fiji software (60 (link)). For cellular quantification of z-stacked images, at least 5 images or 200 cells in total were assessed per sample.

Callaway D.A., Penkala I.J., Zhou S., Knowlton J.J., Cardenas-Diaz F., Babu A., Morley M.P., Lopes M., Garcia B.A, & Morrisey E.E. (2024). TGF-β controls alveolar type 1 epithelial cell plasticity and alveolar matrisome gene transcription in mice. The Journal of Clinical Investigation, 134(6), e172095.

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