Human α syntrophin cdna

Human Type I PDZ GPCR cDNAs were purchased from the Missouri S&T cDNA resource center (cdna.org) or cloned from human brain cDNA library (provided by Professor Ning Zheng, HHMI, University of Washington Department of Pharmacology). Human α-syntrophin cDNA was purchased from OriGene Technologies, (Rockville, MD, USA). Human FLAG-Scribble was provided by Professor Jon Huibregtse (Department of Molecular Sciences, University of Texas at Austin). cDNAs were subcloned into pGlue (provided by Professor Randall T. Moon, HHMI, University of Washington Department of Pharmacology) using In-Fusion HD cloning technology (Clontech).
(R)-(−)-phenylephrine hydrochloride (P6126) was purchased from Sigma (St Louis, MO, USA). Anti-FLAG antibodies were purchased from Sigma (2368). Alexa fluor 633 goat-anti Rabbit IgG (A-21070) and Alexa fluor 568 Goat anti-rat (A-11011) antibodies from Life Technologies. Topro-3 iodide (T3605) is from Life Technologies. Rabbit polyclonal α-syntrophin (H-65, sc-50460), and rabbit polyclonal Scrib (H-300, sc-28737) antibodies from Santa Cruz Biotechnology. Mouse monoclonal Anti-syntrophin (1351, ab11425) antibody and rabbit polyclonal anti-Myc tag antibody (ab9106) from Abcam (Cambridge, MA, USA). Anti-HA mouse mAb (6E2, #2367) from Cell Signaling (Danvers, MA, USA). IRdye 680 goat antimouse IgG and IRdye 800cw goat antirabbit IgG from Li-Cor (Lincoln, NE, USA).