Serum glucose was measured using biochemical assay kits (Pars Azmoon, Iran). Insulin levels of serum and islet secretion samples were evaluated using ELISA assays kits (Monobind, USA) (The sensitivity of hormone detection per assay tube was 0.182 µIU/ml). HOMA-IR (homeostasis model assessment-estimated insulin resistance) was calculated according to the following formula: fasting insulin (µIU/dL) × fasting glucose (mg/dL)/405 [16 ]. Lipid profile and hepatic enzymes in serum samples were measured using biochemical assay kits (Pars Azmoon, Iran) and an Autoanalyzer device (BT3000, Italy).
Elisa assays kits
Manufactured by Monobind
Sourced in United States
ELISA assay kits are laboratory equipment used to detect and measure specific proteins, hormones, or other molecules in a sample. They utilize the principles of enzyme-linked immunosorbent assay (ELISA) to quantify the target analyte through a color change reaction. The kits provide all the necessary components, including reagents and microplates, to perform the analysis in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using elisa assays kits
1
Serum Glucose, Insulin, and Lipid Evaluation
2
Insulin Resistance Evaluation in Animal Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Twenty four hours after the last D-galactose and extract administrations, animals were sacrificed under deep anesthesia by intraperitoneal injection of ketamine-xylazine mixture (70:10 mg/kg) and blood samples were obtained by cardiac puncture. Blood samples were centrifuged at 3000 rpm for 15 min to harvest their serum contents. Then, all samples maintained at −20 °C until hormonal assessment(13 (link)). Glucose was measured by biochemical assay kits (Pars Azmoon, Iran) and serum insulin levels were determined by ELISA assays kits (Monobind, USA) (The sensitivity of hormone detection per assay tube was 0.182 μIU/mL). Further, homeostasis model assessment-estimated insulin resistance (HOMA-IR) was calculated according to the following formula(14 (link)).
HOMA-IR = fasting insulin (μIU/dL) × fasting glucose (mg/dL)/ 405.
HOMA-IR = fasting insulin (μIU/dL) × fasting glucose (mg/dL)/ 405.
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