Ab84828

Immunohistochemical Staining performed after being dewaxed of mice tissue sections. Sections permeabilized with 0.1% Triton X-100, and blocked with 10% goat serum at room temperature for 1 h. Samples were incubated with anti-rabbit SOCS3 antibody (for;1:500, ab16030, Abcam, USA), anti-rabbit MMP9 (1:500, ab76003, Abcam, Cambridge, MA, USA), anti-rabbit-AQP9 antibody (1:600, ab84828, Abcam, Cambridge, MA, USA), and anti-rabbit-MPO antibody (1:600, EPR20257, Abcam, Cambridge, MA, USA) at 4°C overnight. On the 2nd day, slides were incubated with goat anti-rabbit/rabbit FITC secondary antibody for 1h at room temperature. The nuclei were dyed by 4′,6-diamidino-2-phenylindole (DAPI) for 5min (4′,6-diamidino-2-phenylindole, Vector, ZsBio, Beijing, China). Images were obtained using Zeiss fluorescence microscope (Germany) and Nikon microscope (Nikon, Tokyo, Japan). At least five fields for each coverslip were captured. The number of cells, antigen distribution, and cellular fluorescence intensity that represented antigenicity were measured using the captured images. The image analysis was performed with ImageJ (NIH, Bethesda, MD, USA).