Cd62l pe clone mel 14

Manufactured by BD

CD62L-PE (clone MEL-14) is a fluorescently labeled antibody that binds to the CD62L (L-selectin) protein on the surface of cells. CD62L is involved in the homing and migration of lymphocytes. The PE (Phycoerythrin) fluorescent label allows for the detection and analysis of CD62L-expressing cells using flow cytometry.

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CD62L (98 citations) CD62L-PE (32 citations) CD62L (MEL-14; (19 citations) Clone 14 (13 citations) MEL-14 (9 citations) CD62L (clone MEL‐14, (7 citations)

2 protocols using cd62l pe clone mel 14

Flow Cytometric Analysis of Murine Lymphocytes

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Spleen tissue was gently mashed through a 70 µm nylon membrane filter in lymphocyte separation medium to obtain single cell suspensions. Lymphocytes were collected following centrifugation. Following cell count and dilution, lymphocytes were treated with anti-mouse CD16/CD32 antibodies (Mouse BD Fc Block, BD Biosciences) for 15 min and then stained with CD4-FITC (clone RM4-5, eBioscience), CD8-PerCP-Cy5.5 (clone 53-6.7, eBioscience), CD62L-PE (clone MEL-14, BD Biosciences), and CD44-APC (clone IM7, eBioscience) antibodies. The intracellular staining of TLR8 (clone 112H7.15, Dendritics) or BCL2 (clone BCL/10C4, Biolegend) was performed according to the instructions of the manufacturer (Fixation/Permeabilization Solution Kit, BD Biosciences). Following staining, cells were washed with PBS, fixed in 1% formaldehyde, and analyzed on BD Accuri C6 Plus or FACSAria (BD bioscience). Frequency of target cell types was calculated by FlowJo software.

Tang J., Sun M., Shi G., Xu Y., Han Y., Li X., Dong W., Zhan L, & Qin C. (2018). Toll-Like Receptor 8 Agonist Strengthens the Protective Efficacy of ESAT-6 Immunization to Mycobacterium tuberculosis Infection. Frontiers in Immunology, 8, 1972.

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Immunophenotyping Tumor-Infiltrating Cells

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Immunophenotypes of single-cell suspensions from tumors were assessed by fluorescence-activated cell sorting (FACS) flow cytometry. To stain myeloid-derived suppressor cells (MDSCs), antibodies used were CD11b-APC (clone M1/70), Ly6G-FITC (clone 1A8), and Ly6C-PE (clone AL-21; all, BD Biosciences, San Jose, CA). For T cell activation markers, cells were stained with an antibody specific for CD4-PE-Cy7 (clone RM4–5), CD8-PE-Cy7 (clone 53–6.7), CD62L-PE (clone MEL-14; all three, BD Biosciences), or CD44-FITC (clone IM7; Biolegend, San Diego, CA). After incubation on ice for 30 minutes, cells were washed and then fixed in PBS containing 1% formalin for analysis on an LSRII flow cytometer (BD Biosciences).

Kim M.S., Ma S., Chelariu-Raicu A., Leuschner C., Alila H.W., Lee S., Coleman R.L, & Sood A.K. (2020). Enhanced immunotherapy with LHRH-R targeted lytic peptide in ovarian cancer. Molecular cancer therapeutics, 19(11), 2396-2406.

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