Eclipse ts2 fl microscope
The Eclipse Ts2-FL microscope is a high-quality research-grade microscope designed for fluorescence imaging applications. It features a stable and ergonomic design, providing reliable performance for a wide range of microscopy techniques.
Lab products found in correlation
12 protocols using eclipse ts2 fl microscope
Neuronal Differentiation of Mouse P19 Cells
Trilineage Differentiation Potential of MSCs
Measuring MSC Proliferation and Metabolic Activity
Using Fiji ImageJ software, the images obtained before passaging were uniformly enhanced in contrast, a background subtraction was done and images were binarized, with adapted thresholds for each image, and the confluent area within each image was measured. In addition, MSC metabolic activity was measured at day 1 and 5 using a tetrazolium compound (MTS) assay according to the manufacturer's instructions (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega, Mannheim, Germany). The mean absorbance at day 5 was divided by the mean absorbance at day 1 as an indicator for metabolic activity.
Diverse Cellular Stress Responses
Quantifying Cell Adhesion in HeLa Cells
of 25 × 103 cells/well were pretreated with an inhibitor
for 15 min before being plated onto a gelatin-coated 24-well plate
and were incubated for 30 min at 37 °C. Further, 4% paraformaldehyde
was used to fix the attached cells to the surface.63 (link) A Nikon ECLIPSE
TS2-Fl microscope was used to capture the microphotographs,
and the number of attached cells was quantified using NIH Image J
software. Based on these images, differential analysis was performed
for treated and untreated conditions.63 (link)
Microfluidic Emulsion Imaging Protocol
Microscopic Examination of Cell Morphology
Wound Healing Assay for Cell Migration
Cell Viability Evaluation: MTT and Crystal Violet
For crystal violet staining, cells were fixed in 6% formaldehyde and incubated with 0.1% crystal violet for 15 min. Unbound staining was then washed with H2O, and cells were imaged using a Nikon Eclipse Ts2-FL microscope.
Conditioned Media Effects on Cell Migration
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